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Recombinant Arabidopsis thaliana Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM3 (BAM3), partial

Recombinant Arabidopsis thaliana Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM3 (BAM3), partial

Code	CSB-YP527727DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP527727DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP527727DOA-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP527727DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP527727DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

BAM3

Uniprot No.

O65440

Alternative Names

BAM3; At4g20270; F1C12.190Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM3; EC 2.7.11.1; Protein BARELY ANY MERISTEM 3

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Necessary for male gametophyte development, as well as ovule specification and function. Required for the development of high-ordered vascular strands within the leaf and a correlated control of leaf shape, size and symmetry. LRR-rich receptor-like kinase (LRR-RLK) involved in the perception of CLE45 peptide ligand which mediates root growth inhibition by repressing protophloem differentiation; this mechanism requires CRN. BRX, BAM3, and CLE45 act together to regulate the transition of protophloem cells from proliferation to differentiation, thus impinging on postembryonic growth capacity of the root meristem. Necessary for CLE45 peptide-triggered accumulation of MAKR5 in developing sieve elements.

Gene References into Functions

BAM3 protein levels remained unchanged under cold stress. Sodium nitroprusside, which releases NO, caused a decline in BAM3.Treatment of recombinant BAM3 with GSNO caused significant, dose-dependent inhibition of BAM3 activity. In vitro BAM3 inhibition was caused by glutathionylation at cysteine 433. The decrease in BAM3 activity under cold stress may be a result of post-translational nitroso- or glutathionylation. PMID: 29309132
study uncovers BAM3 as the CLE45 receptor in the root and biochemically map its peptide binding surface; CRN stabilizes BAM3 expression and is required for BAM3-mediated CLE45 signaling PMID: 28607033
Amino acid composition of BAM1 and BAM3 orthologs reflected differences that are consistent with known adaptations of proteins from heat- and cold-adapted organisms, suggesting that the day- and night-active enzymes have undergone thermal adaptation. PMID: 25293962
Suppression of Arabidopsis protophloem differentiation and root meristem growth by CLE45 requires the receptor-like kinase BAM3. PMID: 23569225

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Tissue Specificity

Expressed in seedlings, roots, leaves, stems, inflorescences, flowers and siliques. In roots, confined to protophloem and sieve element precursor cells.

Database Links

KEGG: ath:AT4G20270

STRING: 3702.AT4G20270.1

UniGene: At.32731

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