Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BGLU26 Polyclonal antibody

Uniprot No.

O64883

Target Names

BGLU26

Alternative Names

BGLU26 antibody; PEN2 antibody; At2g44490 antibody; F4I1.30 antibody; Beta-glucosidase 26 antibody; peroxisomal antibody; AtBGLU26 antibody; EC 3.2.1.21 antibody; Protein PENETRATION 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana BGLU26 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Possesses beta-glucosidase activity toward 4-methyl-umbelliferyl-beta-D-glucoside in vitro. Possesses myrosinase activity toward indol-3-yl-methylglucosinolate (I3M) and 4-methoxy-indol-3-yl-methylglucosinolate (4MO-I3M) in vivo. Component of an inducible preinvasion resistance mechanism that prevents penetration of the nonhost fungal species B.graminis and E.pisi. Involved in indole glucosinolate (IGS) activation during pattern-triggered immunity (PTI). Functions as myrosinase for the breakdown of flg22-triggered IGS. Required for both callose deposition and glucosinolate activation during pathogen-triggered resistance. During fungal attack, required for IGS activation that mediates broad-spectrum antifungal defense.

Gene References into Functions

PEN2 is a tail-anchored protein with dual-membrane targeting to peroxisomes and mitochondria and that PEN2 has the capacity to form homo-oligomer complexes. PMID: 26721862
COR suppresses an salicylic acid (SA)-independent pathway contributing to callose deposition by reducing accumulation of an indole glucosinolate upstream of the activity of the PEN2 myrosinase. PMID: 23204405
PEN2 restricts pathogen entry of two ascomycete powdery mildew fungi that in nature colonize grass and pea species; the PEN2 glycosyl hydrolase localizes to peroxisomes and acts as a component of an inducible preinvasion resistance mechanism. [PEN2] PMID: 16293760
CYP81F2 gene encodes a P450 monooxygenase that is essential for the pathogen-induced accumulation of 4-methoxyindol-3-ylmethylglucosinolate, which in turn is activated by the atypical PEN2 myrosinase for antifungal defense PMID: 19095900
Double mutant analysis indicated that AGB1 and PMR5 contribute to PEN2-mediated preinvasion resistance to M. oryzae in A. thaliana, suggesting a complex genetic network regulating the resistance. PMID: 19810803

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Subcellular Location

Peroxisome.

Protein Families

Glycosyl hydrolase 1 family

Database Links

KEGG: ath:AT2G44490

STRING: 3702.AT2G44490.1

UniGene: At.36752

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BGLU26 Antibody

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