Recombinant Arabidopsis thaliana Beta-glucosidase 26, peroxisomal (BGLU26), partial

Code CSB-YP530168DOA
MSDS
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Source Yeast
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Code CSB-EP530168DOA
MSDS
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Source E.coli
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Code CSB-EP530168DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP530168DOA
MSDS
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Source Baculovirus
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Code CSB-MP530168DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BGLU26
Uniprot No.
Alternative Names
BGLU26; PEN2; At2g44490; F4I1.30; Beta-glucosidase 26; peroxisomal; AtBGLU26; EC 3.2.1.21; Protein PENETRATION 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Possesses beta-glucosidase activity toward 4-methyl-umbelliferyl-beta-D-glucoside in vitro. Possesses myrosinase activity toward indol-3-yl-methylglucosinolate (I3M) and 4-methoxy-indol-3-yl-methylglucosinolate (4MO-I3M) in vivo. Component of an inducible preinvasion resistance mechanism that prevents penetration of the nonhost fungal species B.graminis and E.pisi. Involved in indole glucosinolate (IGS) activation during pattern-triggered immunity (PTI). Functions as myrosinase for the breakdown of flg22-triggered IGS. Required for both callose deposition and glucosinolate activation during pathogen-triggered resistance. During fungal attack, required for IGS activation that mediates broad-spectrum antifungal defense.
Gene References into Functions
  1. PEN2 is a tail-anchored protein with dual-membrane targeting to peroxisomes and mitochondria and that PEN2 has the capacity to form homo-oligomer complexes. PMID: 26721862
  2. COR suppresses an salicylic acid (SA)-independent pathway contributing to callose deposition by reducing accumulation of an indole glucosinolate upstream of the activity of the PEN2 myrosinase. PMID: 23204405
  3. PEN2 restricts pathogen entry of two ascomycete powdery mildew fungi that in nature colonize grass and pea species; the PEN2 glycosyl hydrolase localizes to peroxisomes and acts as a component of an inducible preinvasion resistance mechanism. [PEN2] PMID: 16293760
  4. CYP81F2 gene encodes a P450 monooxygenase that is essential for the pathogen-induced accumulation of 4-methoxyindol-3-ylmethylglucosinolate, which in turn is activated by the atypical PEN2 myrosinase for antifungal defense PMID: 19095900
  5. Double mutant analysis indicated that AGB1 and PMR5 contribute to PEN2-mediated preinvasion resistance to M. oryzae in A. thaliana, suggesting a complex genetic network regulating the resistance. PMID: 19810803

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Subcellular Location
Peroxisome.
Protein Families
Glycosyl hydrolase 1 family
Database Links

KEGG: ath:AT2G44490

STRING: 3702.AT2G44490.1

UniGene: At.36752

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