BI-1 Antibody

Code CSB-PA873243XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BI-1 Polyclonal antibody
Uniprot No.
Target Names
BI-1
Alternative Names
BI-1; At5g47120; K14A3.7; Bax inhibitor 1; AtBI-1; BI-1
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana BI-1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Suppressor of apoptosis. Modulator of endoplasmic reticulum stress-mediated programmed cell death. Involved in methyl jasmonate-induced leaf senescence through regulating cytoplasmic calcium level.
Gene References into Functions
  1. BI-1 affects cell death-associated components localized in sphingolipid-enriched microdomains of the plasma membrane. PMID: 26297139
  2. AtBI-1 contributes to synthesis of sphingolipids during cold stress by interacting with AtSLD1, AtFAH1, AtSBH2 and AtADS2. PMID: 24687220
  3. Arabidopsis thaliana BI-1-interacting proteins were identified. PMID: 23782494
  4. Overexpression of AtBI-1 increases the amount of 2-hydroxy fatty acids. PMID: 19054355
  5. BAX INHIBITOR 1 is an attenuator for cell death progression triggered by both biotic and abiotic types of cell death signals in Arabidopsis. PMID: 16507080
  6. The expression of AtBI-1, which suppresses Bax-induced cell death, in the tapetum at the tetrad stage inhibits tapetum degeneration and subsequently results in pollen abortion, while activation of AtBI-1 at the later stage does not. PMID: 16565524
  7. A study evaluating the effect of AtBI-1 on calcium homeostasis is reported. PMID: 17142482
  8. BI-1 requires normal electron transport activity to suppress cell death in yeast PMID: 17825821
  9. Results indicate that the loss of calmodulin binding reduces the cell death suppressor activity of Bax inhibitor-1 in planta. PMID: 19674971

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
BI1 family
Tissue Specificity
Expressed in root tips, root vasculature, flower tissues, including stamens and sepals, and in the base of siliques. Not detected in mature leaves.
Database Links

KEGG: ath:AT5G47120

STRING: 3702.AT5G47120.1

UniGene: At.24669

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