Product Details

Full Product Name

Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) tbxta Polyclonal antibody

Uniprot No.

Q07998

Target Names

tbxta

Alternative Names

tbxta antibody; ntl antibody; ntla antibody; tT-box transcription factor T-A antibody; Brachyury protein homolog-A antibody; No tail protein A-A antibody; No tail protein-A antibody; Protein T homolog-A antibody; T-box protein ZfT-A antibody; Zf-T-A antibody

Raised in

Rabbit

Species Reactivity

Danio rerio (Zebrafish) (Brachydanio rerio)

Immunogen

Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) tbxta protein

Immunogen Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in the transcriptional regulation of genes required for mesoderm differentiation, including itself. Indispensable for the formation of the notochord and the tail structure. Functions together with tbx16/spadetail in development of trunk and tail mesoderm. Functions by itself early in development to repress medial floor plate and promote notochord fate but at later times, functions together with tbx16/spadetail to promote medial floor plate formation. Acts in a parallel pathway to, but cooperates with, non-canonical wnt-signaling during tail formation. Required for the morphogenesis of Kupffer's vesicle and regulates left-right asymmetry.

Gene References into Functions

Ntla and Spt do not differentially regulate medial floor plate (MPF) induction along the anterior-posterior axis; rather, the T-box genes act redundantly within margin derived cells to promote the posterior extension of MFP progenitors. PMID: 25781211
observed normal cardiac convergence in spt(-/-) and ntl(-/-) embryos implying that these novel roles of spt and ntl in PGP migration cannot be explained simply by generalised effects on midline convergence PMID: 23860396
As a proof of principle, it has dynamically profiled No tail a (Ntla)-dependent genes at different stages of axial mesoderm development in zebrafish, discovering discrete sets of transcripts. PMID: 22286130
embryonic mesodermal progenitors uniquely establish their own niche--with Brachyury being essential for creating a domain of high Wnt and low RA signaling--rather than having a niche created by separate support cells. PMID: 21159819
Data demonstrate that the nodal-independent regulation of ntl is essential for tail formation in zebrafish. PMID: 20215349
ntl function is required to repress medial floor plate and promote notochord fate in cells of the wild-type notochord domain and spt and ntl together are required non cell-autonomously for medial floor plate formation. PMID: 12091302
intronless ntl gene, ctl has a physiological function in zebrafish PMID: 12804607
Interplay between FGF, one-eyed pinhead (zoep), and T-box transcription factors spt and ntl during zebrafish posterior development. PMID: 14651930
no tail gene (ntl), a key gene participating in the formation of normal tail, was used as a target for ribonuclease (RNase) P-mediated gene disruption in zebrafish in vivo. PMID: 17294249
investigate whether loss of Brachyury/Notail or Tbx16/Spadetail disrupts the balance of Nodal ligands (Southpaw) and antagonists (Charon) around Kupffers Vesicle PMID: 17654709
Ntl is necessary for KV lumen formation. PMID: 17765888
the endoplasmic reticulum Ca(2+) pump has a role in expression of no tail (ntl) and left-right dynein related (lrdr) in the dorsal forerunner cells during gastrulation PMID: 18359204
Regulation of canonical Wnt signaling by ntl is essential for posterior mesoderm formation. PMID: 18606146
Ntl regulates a downstream network of other transcription factors and identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. PMID: 19225104

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Subcellular Location

Nucleus.

Tissue Specificity

First expressed at the dorsal side of the blastula embryo. Expressed in the germ ring, shield and chordamesoderm during gastrulation and is restricted to the notochord and tailbud during somitogenesis (at protein level).

Database Links

KEGG: dre:30399

STRING: 7955.ENSDARP00000005351

UniGene: Dr.1468

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tbxta Antibody

Product Details

Customer Reviews and Q&A

Target Background