brm Antibody

Code CSB-PA338671XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) brm Polyclonal antibody
Uniprot No.
Target Names
brm
Alternative Names
brm antibody; CG5942 antibody; ATP-dependent helicase brm antibody; EC 3.6.4.12 antibody; Homeotic gene regulator antibody; Protein brahma antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) brm protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transcriptional regulator. Acts as a coactivator, assisting one or more dedicated transcriptional activators of ANTC and BXC homeotic gene clusters. Can counteract the repressive effect of Polycomb protein. ATPase subunit of the Brahma complex, a multiprotein complex which is the equivalent of the yeast SWI/SNF complex and acts by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. This complex can both serve as a transcriptional coactivator or corepressor, depending on the context. In type II neuroblast lineage, as part of the Brm remodeling complex, suppresses the formation of ectopic neuroblasts probably through interaction with erm and HDAC3.
Gene References into Functions
  1. Study characterizes the BRAHMA (BRM) chromatin-remodeling protein in Drosophila in the context of circadian clock regulation. By dissecting its catalytic vs. non-catalytic activities, we propose a model in which the non-catalytic activity of BRM functions to recruit repressive factors to limit the transcriptional output of CLK during the active phase of circadian transcription. PMID: 26926115
  2. This paper identified the Brahma (Brm) complex as a regulator of the Drosophila clock PMID: 26132408
  3. Data indicate that brahma protein (Brm) in the form of the PBAP complex functions in the germline stem cells (GSCs) fate regulation. PMID: 25068272
  4. Brahma functions as a critical regulator of Hippo signaling during tissue growth and plays an important role in the feedback loop between Crb and Yki. PMID: 25496831
  5. Brm-HDAC3-Erm repressor complex suppresses dedifferentiation of intermediate neural progenitors back into type II neuroblasts. PMID: 24618901
  6. The results show that Brm knockdown leads to nucleosome occupancy changes throughout the entire genome with a bias in occupancy decrease. PMID: 25081211
  7. Results show that the levels of BRM affect specifically the abundance of a trans-spliced mod(mdg4) mRNA isoform in both S2 cells and larvae. PMID: 24526065
  8. TnaA physically interacts with the SUMO E2 conjugating enzyme Ubc9, and with the BRM complex subunit Osa. PMID: 23620817
  9. Amongst the dominant suppressors of Drosophila cyclin E (DmcycE(JP)), identified brahma (brm) and moira (mor), which encode conserved core components of the Drosophila Brm complex PMID: 12093739
  10. complex facilitates global transcription by RNA polymerase II PMID: 12356740
  11. Consistent with this view, SNR1(E1) is stably associated with other components of the Brm complex at the restrictive temperature. PMID: 12482982
  12. SNR1 is essential for some, but not all Brm functions, and it likely serves as an optional subunit, directing Brm complex activity to specific gene loci or cellular processes. PMID: 12645932
  13. SNR1 (SNF5-Related-1), a highly conserved subunit of the Brm complex, is required to restrict complex activity during the development of wing vein and intervein cells. PMID: 15013794
  14. The abundant HMG box proteins HMGB AND HGMD participate in Brahma-dependent chromatin remodeling at a specific subset of Brahma-dependent promoters. PMID: 16299391
  15. This study reports that the Brm complex assists in coordinating hormone-dependent transcription regulation of the Eig genes. PMID: 16990270
  16. brahma chromatin-remodeling complexes interaction with gammaTub23C PMID: 18780727

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Subcellular Location
Nucleus.
Database Links

KEGG: dme:Dmel_CG5942

STRING: 7227.FBpp0303193

UniGene: Dm.5356

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