BRR2 Antibody

Code CSB-PA327153XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) BRR2 Polyclonal antibody
Uniprot No.
Target Names
BRR2
Alternative Names
BRR2 antibody; RSS1 antibody; SNU246 antibody; YER172C antibody; SYGP-ORF66Pre-mRNA-splicing helicase BRR2 antibody; EC 3.6.4.13 antibody; Protein Snu246 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) BRR2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.
Gene References into Functions
  1. Data show that the truncation of the first 247 amino acids of the N terminus in Bad Response to Refrigeration 2 (Delta247-Brr2) weakened RNA binding, reduced helicase activity, and reduced ATPase activity compared with wild type Brr2. PMID: 27072132
  2. the N-terminal region autoinhibits Brr2 via substrate competition and conformational clamping PMID: 26637280
  3. The N-terminal domain iscritical for retaining U5 and U6 snRNPs during/after spliceosomal activation through its interaction with snRNAs and other spliceosomal proteins, revealing a role of Brr2 in spliceosomal activation in addition to U4/U6 unwinding. PMID: 25670679
  4. structure explains why Aar2 and Brr2 are mutually exclusive and provides important insights into the assembly of U5 snRNP PMID: 23727230
  5. function for Brr2p in driving conformational rearrangements that lead to competence for the second step of splicing PMID: 23124065
  6. the mechanism whereby Brr2 unwinds U4/U6 and show how this activity is potentially regulated prior to spliceosome activation PMID: 23124066
  7. Results suggest that Brr2 shares a similar helicase mechanism with Hel308, and demonstrate that Hel308-II interacts with Prp8 and Snu114 in vitro and in vivo. PMID: 19525970

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Subcellular Location
Nucleus.
Protein Families
Helicase family, SKI2 subfamily
Database Links

KEGG: sce:YER172C

STRING: 4932.YER172C

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