Home
Protein
Recombinant Protein
Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing helicase BRR2 (BRR2), partial

Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing helicase BRR2 (BRR2), partial

Code	CSB-YP327153SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP327153SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP327153SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP327153SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP327153SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

BRR2

Uniprot No.

P32639

Alternative Names

BRR2; RSS1; SNU246; YER172C; SYGP-ORF66Pre-mRNA-splicing helicase BRR2; EC 3.6.4.13; Protein Snu246

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.

Gene References into Functions

Data show that the truncation of the first 247 amino acids of the N terminus in Bad Response to Refrigeration 2 (Delta247-Brr2) weakened RNA binding, reduced helicase activity, and reduced ATPase activity compared with wild type Brr2. PMID: 27072132
the N-terminal region autoinhibits Brr2 via substrate competition and conformational clamping PMID: 26637280
The N-terminal domain iscritical for retaining U5 and U6 snRNPs during/after spliceosomal activation through its interaction with snRNAs and other spliceosomal proteins, revealing a role of Brr2 in spliceosomal activation in addition to U4/U6 unwinding. PMID: 25670679
structure explains why Aar2 and Brr2 are mutually exclusive and provides important insights into the assembly of U5 snRNP PMID: 23727230
function for Brr2p in driving conformational rearrangements that lead to competence for the second step of splicing PMID: 23124065
the mechanism whereby Brr2 unwinds U4/U6 and show how this activity is potentially regulated prior to spliceosome activation PMID: 23124066
Results suggest that Brr2 shares a similar helicase mechanism with Hel308, and demonstrate that Hel308-II interacts with Prp8 and Snu114 in vitro and in vivo. PMID: 19525970