CALS5 Antibody

Code CSB-PA666784XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CALS5 Polyclonal antibody
Uniprot No.
Target Names
CALS5
Alternative Names
CALS5 antibody; GSL2 antibody; LAP1 antibody; At2g13680 antibody; F13J11.3 antibody; T10F5.22Callose synthase 5 antibody; EC 2.4.1.34 antibody; 1,3-beta-glucan synthase antibody; Protein GLUCAN SYNTHASE-LIKE 2 antibody; Protein LESS ADHERENT POLLEN 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CALS5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for the formation of the callose wall separating the tetraspores (interstitial wall) and surrounding the pollen mother cells (peripheral wall). Required for exine formation on pollen wall. May be involved in callose synthesis during pollen tube growth. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals.
Gene References into Functions
  1. Cyclin-dependent kinase-G1 regulates pre-mRNA splicing of CalS5. PMID: 23404887
  2. When expressed in cultured tobacco BY-2 cells, CalS5 was found to be present in the plasma membrane and the Golgi-related endomembranes, and resulted in callose accumulation in the cell wall and altered cell wall permeability. PMID: 22195570
  3. CalS5 encodes a callose synthase which is responsible for the synthesis of callose deposited at the primary cell wall of meiocytes, tetrads and microspores, and the expression of this gene is essential for exine formation in pollen wall. [CalS5] PMID: 15842618
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Glycosyltransferase 48 family
Database Links

KEGG: ath:AT2G13680

STRING: 3702.AT2G13680.1

UniGene: At.52728

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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