Recombinant Arabidopsis thaliana Callose synthase 5 (CALS5), partial

Code CSB-YP666784DOA
MSDS
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Source Yeast
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Code CSB-EP666784DOA
MSDS
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Source E.coli
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Code CSB-EP666784DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP666784DOA
MSDS
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Source Baculovirus
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Code CSB-MP666784DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CALS5
Uniprot No.
Alternative Names
CALS5; GSL2; LAP1; At2g13680; F13J11.3; T10F5.22Callose synthase 5; EC 2.4.1.34; 1,3-beta-glucan synthase; Protein GLUCAN SYNTHASE-LIKE 2; Protein LESS ADHERENT POLLEN 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for the formation of the callose wall separating the tetraspores (interstitial wall) and surrounding the pollen mother cells (peripheral wall). Required for exine formation on pollen wall. May be involved in callose synthesis during pollen tube growth. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals.
Gene References into Functions
  1. Cyclin-dependent kinase-G1 regulates pre-mRNA splicing of CalS5. PMID: 23404887
  2. When expressed in cultured tobacco BY-2 cells, CalS5 was found to be present in the plasma membrane and the Golgi-related endomembranes, and resulted in callose accumulation in the cell wall and altered cell wall permeability. PMID: 22195570
  3. CalS5 encodes a callose synthase which is responsible for the synthesis of callose deposited at the primary cell wall of meiocytes, tetrads and microspores, and the expression of this gene is essential for exine formation in pollen wall. [CalS5] PMID: 15842618
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Glycosyltransferase 48 family
Database Links

KEGG: ath:AT2G13680

STRING: 3702.AT2G13680.1

UniGene: At.52728

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