Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) ygcB Polyclonal antibody

Uniprot No.

P38036

Target Names

ygcB

Alternative Names

ygcB antibody; cas3 antibody; b2761 antibody; JW2731 antibody; CRISPR-associated endonuclease/helicase Cas3 antibody; EC 3.1.-.- antibody; EC 3.6.4.- antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) CAS3 protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The CAS3 polyclonal antibody is generated by injecting the recombinant Escherichia coli (strain K12) CAS3 protein into a rabbit, which triggers the production of antibodies that specifically target this antigen. To increase antibody production, a booster injection is administered. The resulting CAS3 polyclonal antibodies in the rabbit's serum are collected and purified using protein A/G. Rigorous testing through ELISA and WB assays has verified that the CAS3 antibody specifically recognizes Escherichia coli (strain K12) CAS3 protein.

The Escherichia coli (strain K12) CAS3 protein, also known as CRISPR-associated protein 3, mainly participates in the CRISPR-Cas system. CAS3 protein plays a crucial role in the bacterial adaptive immune system by targeting and degrading foreign genetic material, such as viral DNA or plasmids. It acts as a nuclease enzyme that cleaves the invading DNA, thereby providing defense against viral infections and horizontal gene transfer.

Target Background

Function

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity.; Acts as an endonuclease, a 3'-5'exonuclease, and an ATP-dependent dsDNA helicase. Anneals and unwinds R-loops (in which crRNA binds the target DNA, displacing the noncomplementary strand). Unwinding requires ATP, annealing does not. Required along with the Cascade complex for resistance to bacteriophage lambda infection as well as the ability to cure CRISPR-encoding high-copy number plasmid. A Cas3-CasA fusion protein purified with the Cascade complex nicks target plasmid in the presence but not absence of Mg(2+), and degrades plasmid fully in the presence of Mg(2+) and ATP, suggesting the helicase activity is required for complete degradation.

Gene References into Functions

Cas3-derived target DNA degradation fragments fuel primed CRISPR adaptation. PMID: 27546790
study observed that Cas3 promoted runaway ColE1 plasmid replication, requiring Cas3 helicase activity; Cas3 expression antagonized RNaseHI, observed as a concatamer phenotype, but also required RNaseHI for its ability to stimulate plasmid copy number PMID: 23406879
Cas3 is involved in DNA degradation and invader neutralization. PMID: 22521689
Study discuss possible overlapping roles of Cas3 and Cascade in E. coli, and the apparently antagonistic roles of Cas3 catalysing RNA-DNA annealing and ATP-dependent helicase unwinding. PMID: 21699496

Protein Families

CRISPR-associated nuclease Cas3-HD family; CRISPR-associated helicase Cas3 family

Database Links

KEGG: ecj:JW2731

STRING: 316385.ECDH10B_2929

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Code	CSB-PA334357XA01ENV
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: ygcB antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31.8 kDa Observed band size: 32 kDa
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CAS3 Antibody

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