CAX1 Antibody

Code CSB-PA653701XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CAX1 Polyclonal antibody
Uniprot No.
Target Names
CAX1
Alternative Names
CAX1 antibody; RCI4 antibody; At2g38170 antibody; F16M14.10Vacuolar cation/proton exchanger 1 antibody; Ca(2+)/H(+) antiporter CAX1 antibody; Ca(2+)/H(+) exchanger 1 antibody; Protein CATION EXCHANGER 1 antibody; Protein RARE COLD INDUCIBLE 4 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CAX1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Vacuolar cation/proton exchanger (CAX). Translocates Ca(2+) and other metal ions into vacuoles using the proton gradient formed by H(+)-ATPase and H(+)-pyrophosphatase. Involved in ion homeostasis in association with CAX3. May play a role in cold-acclimation response.
Gene References into Functions
  1. Expression of CAX1 and CAX3 occurs in guard cells. Additionally, CAX1 and CAX3 are co-expressed in mesophyll tissue in response to wounding or flg22 treatment, due to the induction of CAX3 expression. The authors demonstrate that CAX1 and CAX3 can form homomeric and heteromeric complexes in plants. PMID: 28645169
  2. cax1/cax3 mutants have a higher apoplastic pH than the wild type, further supporting the hypothesis that there is a defect in auxin import in the cax mutants. PMID: 22932758
  3. Presence of Arabidopsis CAX transporters is important for normal germination and metal homeostasis within the seed. PMID: 23071810
  4. The role of CAX1 in elemental distribution and abundance in Arabidopsis seed. PMID: 22086421
  5. CAX1 and CAX3 mediate a shoot-derived signal that modulates the activity of the root phosphate [P(i)] transporter system, likely in part via posttranslational regulation of PHT1;1 P(i) transporters. PMID: 21546457
  6. Shared and unique aspects of CAX1 and CAX3 calcium transport and regulation are demonstrated. PMID: 21657244
  7. The results indicate that the high expression of the sCAX1 gene reduces cytosolic and apoplastic Ca concentrations, affecting plasma membrane structure and leading to blossom-end rot symptom development in the fruit tissue. PMID: 21464475
  8. CAX1 has a primary role in Ca accumulation in the leaf mesophyll, and is important in controlling apoplastic [Ca2+], stomatal aperture, and growth. PMID: 21258004
  9. Null CAX1 mutants of Arabidopsis have most of the phenotypes associated with tolerance to serpentine soils, especially altered responses to calcium and magnesium. PMID: 15948832
  10. His338 has a role in metal transport activity in the Arabidopsis cation/H+ exchanger CAX1 PMID: 15994298
  11. The functional roles of calcium exchanger 1 (CAX1), a vacuolar H+/Ca2+ transporter, and the closely related transporter CAX3 are reported. PMID: 16055687
  12. plants expressing activated CAX1 variants displayed hypersensitivity to ion imbalances, increased calcium accumulation, heightened concentrations of other minerals, and increased activity of tonoplast-enriched Ca(2+)/H(+) transport PMID: 17898422
  13. Interaction between Arabidopsis Ca2+/H+ exchangers CAX1 and CAX3. PMID: 19098009
  14. analysis of interactions between CAX protein domains through the use of nonfunctional halves of CAX transporters PMID: 19819871

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Subcellular Location
Vacuole membrane; Multi-pass membrane protein. Note=Tonoplast.
Protein Families
Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family, Cation/proton exchanger (CAX) subfamily
Tissue Specificity
Expressed at low levels in leaves, stems and flowers.
Database Links

KEGG: ath:AT2G38170

STRING: 3702.AT2G38170.3

UniGene: At.12459

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