Recombinant Arabidopsis thaliana Vacuolar cation/proton exchanger 1 (CAX1), partial

Code CSB-YP653701DOA
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Source Yeast
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Code CSB-EP653701DOA
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Source E.coli
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Code CSB-EP653701DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP653701DOA
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Source Baculovirus
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Code CSB-MP653701DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CAX1
Uniprot No.
Alternative Names
CAX1; RCI4; At2g38170; F16M14.10Vacuolar cation/proton exchanger 1; Ca(2+)/H(+) antiporter CAX1; Ca(2+)/H(+) exchanger 1; Protein CATION EXCHANGER 1; Protein RARE COLD INDUCIBLE 4
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Vacuolar cation/proton exchanger (CAX). Translocates Ca(2+) and other metal ions into vacuoles using the proton gradient formed by H(+)-ATPase and H(+)-pyrophosphatase. Involved in ion homeostasis in association with CAX3. May play a role in cold-acclimation response.
Gene References into Functions
  1. Expression of CAX1 and CAX3 occurs in guard cells. Additionally, CAX1 and CAX3 are co-expressed in mesophyll tissue in response to wounding or flg22 treatment, due to the induction of CAX3 expression. The authors demonstrate that CAX1 and CAX3 can form homomeric and heteromeric complexes in plants. PMID: 28645169
  2. cax1/cax3 mutants have a higher apoplastic pH than the wild type, further supporting the hypothesis that there is a defect in auxin import in the cax mutants. PMID: 22932758
  3. Presence of Arabidopsis CAX transporters is important for normal germination and metal homeostasis within the seed. PMID: 23071810
  4. The role of CAX1 in elemental distribution and abundance in Arabidopsis seed. PMID: 22086421
  5. CAX1 and CAX3 mediate a shoot-derived signal that modulates the activity of the root phosphate [P(i)] transporter system, likely in part via posttranslational regulation of PHT1;1 P(i) transporters. PMID: 21546457
  6. Shared and unique aspects of CAX1 and CAX3 calcium transport and regulation are demonstrated. PMID: 21657244
  7. The results indicate that the high expression of the sCAX1 gene reduces cytosolic and apoplastic Ca concentrations, affecting plasma membrane structure and leading to blossom-end rot symptom development in the fruit tissue. PMID: 21464475
  8. CAX1 has a primary role in Ca accumulation in the leaf mesophyll, and is important in controlling apoplastic [Ca2+], stomatal aperture, and growth. PMID: 21258004
  9. Null CAX1 mutants of Arabidopsis have most of the phenotypes associated with tolerance to serpentine soils, especially altered responses to calcium and magnesium. PMID: 15948832
  10. His338 has a role in metal transport activity in the Arabidopsis cation/H+ exchanger CAX1 PMID: 15994298
  11. The functional roles of calcium exchanger 1 (CAX1), a vacuolar H+/Ca2+ transporter, and the closely related transporter CAX3 are reported. PMID: 16055687
  12. plants expressing activated CAX1 variants displayed hypersensitivity to ion imbalances, increased calcium accumulation, heightened concentrations of other minerals, and increased activity of tonoplast-enriched Ca(2+)/H(+) transport PMID: 17898422
  13. Interaction between Arabidopsis Ca2+/H+ exchangers CAX1 and CAX3. PMID: 19098009
  14. analysis of interactions between CAX protein domains through the use of nonfunctional halves of CAX transporters PMID: 19819871

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Subcellular Location
Vacuole membrane; Multi-pass membrane protein. Note=Tonoplast.
Protein Families
Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family, Cation/proton exchanger (CAX) subfamily
Tissue Specificity
Expressed at low levels in leaves, stems and flowers.
Database Links

KEGG: ath:AT2G38170

STRING: 3702.AT2G38170.3

UniGene: At.12459

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