CycA Antibody

Code CSB-PA321300XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) CycA Polyclonal antibody
Uniprot No.
Target Names
CycA
Alternative Names
CycA antibody; CG5940G2/mitotic-specific cyclin-A antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) CycA protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the Cdk1 and Cdk2 protein kinases to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.
Gene References into Functions
  1. TARA regulates CycA protein levels and genetically and physically interacts with CycA to promote sleep. PMID: 26096977
  2. mitotic ER reorganization events rely on CycA and that control and timing of nuclear and cytoplasmic events during mitosis PMID: 25689737
  3. Through visualization of double heterozygous embryos, we determines that spir cooperates with CycA for heart cell specification and division. PMID: 22276214
  4. It was shown that CycA accumulate during the last period of endoreplication, both CycA loss and gain of function induce changes in endoreplication dynamics and reduce the number of endocycles, and heterochromatin localisation of ORC2 depends on CycA. PMID: 22223681
  5. Regulation of cyclin A localization downstream of Par-1 function is critical for the centrosome orientation checkpoint in Drosophila male germline stem cells. PMID: 22024320
  6. Effete/APC-mediated degradation of Cyclin A is essential for the maintenance of germline stem cells PMID: 19906849
  7. CycA is directly regulated by Polycomb group proteins, linking them to cell cycle control in vivo. PMID: 16481477
  8. Drosophila cyclin A destruction during mitosis requires both N- and C-terminal residues, emphasizing synergistic action by different parts of the protein that facilitate recognition and degradation. PMID: 17314514

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Protein Families
Cyclin family, Cyclin AB subfamily
Database Links

KEGG: dme:Dmel_CG5940

STRING: 7227.FBpp0075807

UniGene: Dm.2549

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