Recombinant Drosophila melanogaster G2/mitotic-specific cyclin-A (CycA)

Code CSB-YP321300DLU
MSDS
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Source Yeast
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Code CSB-EP321300DLU
MSDS
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Source E.coli
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Code CSB-EP321300DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321300DLU
MSDS
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Source Baculovirus
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Code CSB-MP321300DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CycA
Uniprot No.
Alternative Names
CycA; CG5940G2/mitotic-specific cyclin-A
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-491
Target Protein Sequence
MASFQIHQDM SNKENPGIKI PAGVKNTKQP LAVIGGKAEK NALAPRANFA VLNGNNNVPR PAGKVQVFRD VRNLNVDENV EYGAKKSNVV PVVEQFKTFS VYEDNNDTQV APSGKSLASL VDKENHDVKF GAGQKELVDY DLDSTPMSVT DVQSPMSVDR SILGVIQSSD ISVGTETGVS PTGRVKELPP RNDRQRFLEV VQYQMDILEY FRESEKKHRP KPLYMRRQKD ISHNMRSILI DWLVEVSEEY KLDTETLYLS VFYLDRFLSQ MAVVRSKLQL VGTAAMYIAA KYEEIYPPEV GEFVFLTDDS YTKAQVLRME QVILKILSFD LCTPTAYVFI NTYAVLCDMP EKLKYMTLYI SELSLMEGET YLQYLPSLMS SASVALARHI LGMEMWTPRL EEITTYKLED LKTVVLHLCH THKTAKELNT QAMREKYNRD TYKKVAMMES VEMSKDDFDQ LCEAYNCKQK EDEHQQPDIN TKSNVNLFYK F
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the Cdk1 and Cdk2 protein kinases to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.
Gene References into Functions
  1. TARA regulates CycA protein levels and genetically and physically interacts with CycA to promote sleep. PMID: 26096977
  2. mitotic ER reorganization events rely on CycA and that control and timing of nuclear and cytoplasmic events during mitosis PMID: 25689737
  3. Through visualization of double heterozygous embryos, we determines that spir cooperates with CycA for heart cell specification and division. PMID: 22276214
  4. It was shown that CycA accumulate during the last period of endoreplication, both CycA loss and gain of function induce changes in endoreplication dynamics and reduce the number of endocycles, and heterochromatin localisation of ORC2 depends on CycA. PMID: 22223681
  5. Regulation of cyclin A localization downstream of Par-1 function is critical for the centrosome orientation checkpoint in Drosophila male germline stem cells. PMID: 22024320
  6. Effete/APC-mediated degradation of Cyclin A is essential for the maintenance of germline stem cells PMID: 19906849
  7. CycA is directly regulated by Polycomb group proteins, linking them to cell cycle control in vivo. PMID: 16481477
  8. Drosophila cyclin A destruction during mitosis requires both N- and C-terminal residues, emphasizing synergistic action by different parts of the protein that facilitate recognition and degradation. PMID: 17314514

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Protein Families
Cyclin family, Cyclin AB subfamily
Database Links

KEGG: dme:Dmel_CG5940

STRING: 7227.FBpp0075807

UniGene: Dm.2549

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