ccpA Antibody

Code CSB-PA326521XA01BRJ
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Product Details

Full Product Name
Rabbit anti-Bacillus subtilis (strain 168) ccpA Polyclonal antibody
Uniprot No.
Target Names
ccpA
Alternative Names
ccpA antibody; alsA antibody; amyR antibody; graR antibody; BSU29740 antibody; Catabolite control protein A antibody; Glucose-resistance amylase regulator antibody
Raised in
Rabbit
Species Reactivity
Bacillus subtilis (strain 168)
Immunogen
Recombinant Bacillus subtilis (strain 168) ccpA protein
Immunogen Species
Bacillus subtilis (strain 168)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Global transcriptional regulator of carbon catabolite repression (CCR) and carbon catabolite activation (CCA), which ensures optimal energy usage under diverse conditions. Interacts with either P-Ser-HPr or P-Ser-Crh, leading to the formation of a complex that binds to DNA at the catabolite-response elements (cre). Binding to DNA allows activation or repression of many different genes and operons.
Gene References into Functions
  1. Thus, degU is induced through activation of autoregulation by a decrease in ClpCP-dependent proteolysis of DegU-P. PMID: 23123903
  2. Study shows that CcpA forms complexes with CodY and RpoA in Bacillus subtilis. PMID: 22512862
  3. The catabolite repression by malate is exerted by the CcpA-HPrK complex. PMID: 22001508
  4. Phosphorylation of Ser46 of HPr or of the homologous Crh protein by HPr kinase and formation of a repressor complex with CcpA are parts of the B. subtilis carbon catabolite repression system. PMID: 20139185
  5. interacts with RNA polymerase to inhibit transcription. PMID: 15773986
  6. Data suggest specific roles for fructose-1,6-bisphosphate and glucose-6-phosphate in enhancing HPrSerP-, but not CrhP-mediated carbon catabolite regulation through CcpA. PMID: 15885105
  7. binds to the ilvB promoter region and stimulates transcription significantly only when CodY is present PMID: 15916605
  8. involved in transcription regulation of ilv-leu operon together with CodY and TnrA regulators of cellular catabolic and anabolic genes. PMID: 15916606
  9. direct repression by CcpA of a phoPR promoter PMID: 16452408
  10. The carbon control protein-A (CcpA) of Bacillus subtilis, primarily concerned with carbon metabolism, influences expression of phosphorus (pho) regulon genes, alkaline phosphatase structural genes, phoA and phoB. PMID: 16491025
  11. A 2.45-A resolution X-ray structure of the binary complex between B. subtilis PserHPr and CcpA was reported and it also showed the Fructose-1, 6-bisphosphate effector binding site. PMID: 16755587
  12. Transcription of the citST operon was repressed in the presence of glucose by the general transcription factor CcpA. PMID: 16842348
  13. ccpA regulates ackA expression PMID: 16995897

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