Recombinant Bacillus subtilis Catabolite control protein A (ccpA)

Code CSB-YP326521BRJ
MSDS
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Source Yeast
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Code CSB-EP326521BRJ
MSDS
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Source E.coli
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Code CSB-EP326521BRJ-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP326521BRJ
MSDS
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Source Baculovirus
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Code CSB-MP326521BRJ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ccpA
Uniprot No.
Alternative Names
ccpA; alsA; amyR; graR; BSU29740; Catabolite control protein A; Glucose-resistance amylase regulator
Species
Bacillus subtilis (strain 168)
Expression Region
1-334
Target Protein Sequence
MSNITIYDVA REANVSMATV SRVVNGNPNV KPTTRKKVLE AIERLGYRPN AVARGLASKK TTTVGVIIPD ISSIFYSELA RGIEDIATMY KYNIILSNSD QNMEKELHLL NTMLGKQVDG IVFMGGNITD EHVAEFKRSP VPIVLAASVE EQEETPSVAI DYEQAIYDAV KLLVDKGHTD IAFVSGPMAE PINRSKKLQG YKRALEEANL PFNEQFVAEG DYTYDSGLEA LQHLMSLDKK PTAILSATDE MALGIIHAAQ DQGLSIPEDL DIIGFDNTRL SLMVRPQLST VVQPTYDIGA VAMRLLTKLM NKEPVEEHIV ELPHRIELRK STKS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Global transcriptional regulator of carbon catabolite repression (CCR) and carbon catabolite activation (CCA), which ensures optimal energy usage under diverse conditions. Interacts with either P-Ser-HPr or P-Ser-Crh, leading to the formation of a complex that binds to DNA at the catabolite-response elements (cre). Binding to DNA allows activation or repression of many different genes and operons.
Gene References into Functions
  1. Thus, degU is induced through activation of autoregulation by a decrease in ClpCP-dependent proteolysis of DegU-P. PMID: 23123903
  2. Study shows that CcpA forms complexes with CodY and RpoA in Bacillus subtilis. PMID: 22512862
  3. The catabolite repression by malate is exerted by the CcpA-HPrK complex. PMID: 22001508
  4. Phosphorylation of Ser46 of HPr or of the homologous Crh protein by HPr kinase and formation of a repressor complex with CcpA are parts of the B. subtilis carbon catabolite repression system. PMID: 20139185
  5. interacts with RNA polymerase to inhibit transcription. PMID: 15773986
  6. Data suggest specific roles for fructose-1,6-bisphosphate and glucose-6-phosphate in enhancing HPrSerP-, but not CrhP-mediated carbon catabolite regulation through CcpA. PMID: 15885105
  7. binds to the ilvB promoter region and stimulates transcription significantly only when CodY is present PMID: 15916605
  8. involved in transcription regulation of ilv-leu operon together with CodY and TnrA regulators of cellular catabolic and anabolic genes. PMID: 15916606
  9. direct repression by CcpA of a phoPR promoter PMID: 16452408
  10. The carbon control protein-A (CcpA) of Bacillus subtilis, primarily concerned with carbon metabolism, influences expression of phosphorus (pho) regulon genes, alkaline phosphatase structural genes, phoA and phoB. PMID: 16491025
  11. A 2.45-A resolution X-ray structure of the binary complex between B. subtilis PserHPr and CcpA was reported and it also showed the Fructose-1, 6-bisphosphate effector binding site. PMID: 16755587
  12. Transcription of the citST operon was repressed in the presence of glucose by the general transcription factor CcpA. PMID: 16842348
  13. ccpA regulates ackA expression PMID: 16995897

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