CCS1 Antibody

Code CSB-PA331142XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CCS1 Polyclonal antibody
Uniprot No.
Target Names
CCS1
Alternative Names
CCS1 antibody; LYS7 antibody; YMR038C antibody; YM9532.03CSuperoxide dismutase 1 copper chaperone antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CCS1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Copper chaperone for apo superoxide dismutase 1 (SOD1). Binds copper ions and delivers them specifically to apo-SOD1.
Gene References into Functions
  1. Data suggest that Ccs1 activates immature Sod1 by delivering copper and facilitating oxidation of intramolecular disulfide bond in Sod1; Ccs1 binding exposes an electropositive cavity and proposed "entry site" for copper ion delivery on the apoenzyme. (Ccs1 = copper chaperone for superoxide dismutase; Sod1 = copper-zinc superoxide dismutase) PMID: 28533431
  2. CCS-dependent copper acquisition and distribution largely occur at membrane interfaces and that this emerging role of the bilayer may reflect a general mechanistic aspect of cellular transition metal ion acquisition. PMID: 24297923
  3. The Mia40/Erv1 disulfide relay system introduces a structural disulfide bond in the copper chaperone Ccs1 between the cysteine residues C27 and C64, thereby promoting mitochondrial import of this unconventional substrate. PMID: 21865601
  4. Results describe the identification of the copper chaperone for superoxide dismutase as a mediator of copper delivery to XIAP in cells. PMID: 20154138
  5. a new relationship between Cu, Zn superoxide dismutase, LYS7, and monitors and participates in meiotic recombination-mediated checkpoint response to replication arrest and DNA damage in Saccharomyces cerevisiae PMID: 16287844

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Subcellular Location
Cytoplasm. Mitochondrion intermembrane space. Note=A small percentage (around 1-5 percent) localizes to the mitochondrial intermembrane space.
Protein Families
CCS1 family
Database Links

KEGG: sce:YMR038C

STRING: 4932.YMR038C

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