Recombinant Saccharomyces cerevisiae Superoxide dismutase 1 copper chaperone (CCS1)

Code CSB-YP331142SVG
MSDS
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Source Yeast
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Code CSB-EP331142SVG
MSDS
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Source E.coli
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Code CSB-EP331142SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP331142SVG
MSDS
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Source Baculovirus
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Code CSB-MP331142SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CCS1
Uniprot No.
Alternative Names
CCS1; LYS7; YMR038C; YM9532.03CSuperoxide dismutase 1 copper chaperone
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-249
Target Protein Sequence
MTTNDTYEAT YAIPMHCENC VNDIKACLKN VPGINSLNFD IEQQIMSVES SVAPSTIINT LRNCGKDAII RGAGKPNSSA VAILETFQKY TIDQKKDTAV RGLARIVQVG ENKTLFDITV NGVPEAGNYH ASIHEKGDVS KGVESTGKVW HKFDEPIECF NESDLGKNLY SGKTFLSAPL PTWQLIGRSF VISKSLNHPE NEPSSVKDYS FLGVIARSAG VWENNKQVCA CTGKTVWEER KDALANNIK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Copper chaperone for apo superoxide dismutase 1 (SOD1). Binds copper ions and delivers them specifically to apo-SOD1.
Gene References into Functions
  1. Data suggest that Ccs1 activates immature Sod1 by delivering copper and facilitating oxidation of intramolecular disulfide bond in Sod1; Ccs1 binding exposes an electropositive cavity and proposed "entry site" for copper ion delivery on the apoenzyme. (Ccs1 = copper chaperone for superoxide dismutase; Sod1 = copper-zinc superoxide dismutase) PMID: 28533431
  2. CCS-dependent copper acquisition and distribution largely occur at membrane interfaces and that this emerging role of the bilayer may reflect a general mechanistic aspect of cellular transition metal ion acquisition. PMID: 24297923
  3. The Mia40/Erv1 disulfide relay system introduces a structural disulfide bond in the copper chaperone Ccs1 between the cysteine residues C27 and C64, thereby promoting mitochondrial import of this unconventional substrate. PMID: 21865601
  4. Results describe the identification of the copper chaperone for superoxide dismutase as a mediator of copper delivery to XIAP in cells. PMID: 20154138
  5. a new relationship between Cu, Zn superoxide dismutase, LYS7, and monitors and participates in meiotic recombination-mediated checkpoint response to replication arrest and DNA damage in Saccharomyces cerevisiae PMID: 16287844

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Subcellular Location
Cytoplasm. Mitochondrion intermembrane space. Note=A small percentage (around 1-5 percent) localizes to the mitochondrial intermembrane space.
Protein Families
CCS1 family
Database Links

KEGG: sce:YMR038C

STRING: 4932.YMR038C

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