Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CDC20 Polyclonal antibody

Uniprot No.

P26309

Target Names

CDC20

Alternative Names

CDC20 antibody; YGL116WAPC/C activator protein CDC20 antibody; Cell division control protein 20 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CDC20 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). At the metaphase-to-anaphase transition, recognizes and binds proteins containing a D-box including the B-type cyclins CLB2 and CLB5, HSL1 and securin PDS1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for sister chromatid separation and disassembly of the mitotic spindle. Target of the spindle checkpoint pathway through participation in the mitotic checkpoint complex (MCC) and the MAD2-CDC20 subcomplex. MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20 until proper attachment of all chromosomes to the spindle.

Gene References into Functions

Small N-terminal regions of both Cdc20 (RxxL-type D box) and Cdc5 (KEN motif) were identified as transferable degradation domains (degrons) that mediated efficient targeting by APC/C-Cdh1 (anaphase-promoting complex/cyclosome-Cdh1). PMID: 25540434
Bub3 has a previously unknown function at the kinetochore in activating APC/C-Cdc20 for normal mitotic progression. PMID: 25987604
Both catalysis of Mad1:C-Mad2 at the kinetochores and Mad2-Cdc20 binding away from kinetochores are required for a proficient checkpoint. PMID: 23000150
study analyzed the role of Cdc20 autoubiquitination in the spindle assembly checkpoint PMID: 22940250
This study finds that the majority of Cdc20 turnover does not involve a second activator molecule but instead depends on in cis Cdc20 autoubiquitination while it is bound to its activator-binding site on the anaphase-promoting complex (APC) core. PMID: 22079111
Mad3 is required for the stable binding of Mad2 to Cdc20 in vivo, which is sufficient to inhibit APC activity and is the most downstream event in spindle checkpoint activation. PMID: 22209528
data suggest important roles of ubiquitination in mediating IGF-1R signaling and degradation PMID: 17406666
model aimed at unifying previous analyses of checkpoint function by focusing on the Mad3-Cdc20 interaction PMID: 17369399

Hide All

Subcellular Location

Nucleus.

Protein Families

WD repeat CDC20/Fizzy family

Database Links

KEGG: sce:YGL116W

STRING: 4932.YGL116W

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CDC20 Antibody

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