CDKF-1 Antibody

Datasheet

Code	CSB-PA529274XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CDKF-1 Polyclonal antibody

Uniprot No.

O80345

Target Names

CDKF-1

Alternative Names

CDKF-1 antibody; CAK1 antibody; At4g28980 antibody; F19B15.10Cyclin-dependent kinase F-1 antibody; CDKF;1 antibody; EC 2.7.11.22 antibody; EC 2.7.11.23 antibody; CDK-activating kinase 1-At antibody; CAK1-At antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CDKF-1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

CDK-activating kinase that modulates CDKD-2 and CDKD-3 activities by phosphorylation of the T-loop. Activates CDKD-2 C-terminal domain (CTD) kinase activity. Activates CDKA-1 probably by phosphorylation. Posseses a CDK kinase activity independently of association with cyclin CYCH1-1. Phosphorylates the CTD of the large subunit of RNA polymerase II.

Gene References into Functions

Ser phosphorylation of Arabidopsis thaliana RNAPII CTD is governed by CYCLIN-DEPENDENT KINASE F;1. PMID: 22547781
CDKF;1 plays a major role in cyclin-dependent kinase and carboxy-terminal domain phosphorylation. PMID: 16856985
CDKF;1 is dispensable for CDKA;1 activation but is essential for maintaining a steady-state level of CDKD;2. CDKF;1 mutants have severe defects in cell division, cell elongation and endoreduplication. PMID: 19368694

Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily

Tissue Specificity

Highly expressed in suspension cell culture. Expressed at low levels in all plant organs.

Database Links

KEGG: ath:AT4G28980

STRING: 3702.AT4G28980.1

UniGene: At.19673

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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CDKF-1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background