Recombinant Arabidopsis thaliana Cyclin-dependent kinase F-1 (CDKF-1)

Code CSB-YP529274DOA
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP529274DOA
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP529274DOA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP529274DOA
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP529274DOA
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
CDKF-1
Uniprot No.
Alternative Names
CDKF-1; CAK1; At4g28980; F19B15.10Cyclin-dependent kinase F-1; CDKF;1; EC 2.7.11.22; EC 2.7.11.23; CDK-activating kinase 1-At; CAK1-At
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-479
Target Protein Sequence
MDKQPATSWS IHTRPEIIAK YEIFERVGSG AYADVYRARR LSDGLIVALK EIFDYQSAFR EIDALTILNG SPNVVVMHEY FWREEENAVL VLEFLRSDLA AVIRDGKRKK KVEGGDGFSV GEIKRWMIQI LTGVDACHRN LIVHRDLKPG NMLISDDGVL KLADFGQARI LMEHDIVASD ENQQAYKLED KDGETSEPPE VIPDYENSPR QGSDGQEREA MSKDEYFRQV EELKAKQVVR DDTDKDSNVH DGDISCLATC TVSEMDDDLG RNSFSYDADE AVDDTQGLMT SCVGTRWFRP PELLYGSTMY GLEVDLWSLG CVFAELLSLE PLFPGISDID QISRVTNVLG NLNEEVWPGC VDLPDYKSIS FAKVESPLGI EGCLPNHSGD VISLLKKLIC YDPASRATTM EMLNDKYLSE EPLPVPVSEL YVPPTMSGPD EDSPRKWNDY REMDSDSDFD GFGPMNVKPT SSGFTIEFP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
CDK-activating kinase that modulates CDKD-2 and CDKD-3 activities by phosphorylation of the T-loop. Activates CDKD-2 C-terminal domain (CTD) kinase activity. Activates CDKA-1 probably by phosphorylation. Posseses a CDK kinase activity independently of association with cyclin CYCH1-1. Phosphorylates the CTD of the large subunit of RNA polymerase II.
Gene References into Functions
  1. Ser phosphorylation of Arabidopsis thaliana RNAPII CTD is governed by CYCLIN-DEPENDENT KINASE F;1. PMID: 22547781
  2. CDKF;1 plays a major role in cyclin-dependent kinase and carboxy-terminal domain phosphorylation. PMID: 16856985
  3. CDKF;1 is dispensable for CDKA;1 activation but is essential for maintaining a steady-state level of CDKD;2. CDKF;1 mutants have severe defects in cell division, cell elongation and endoreduplication. PMID: 19368694
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
Tissue Specificity
Highly expressed in suspension cell culture. Expressed at low levels in all plant organs.
Database Links

KEGG: ath:AT4G28980

STRING: 3702.AT4G28980.1

UniGene: At.19673

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2025 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1