CPK28 Antibody

Datasheet

Code	CSB-PA884278XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CPK28 Polyclonal antibody

Uniprot No.

Q9FKW4

Target Names

CPK28

Alternative Names

CPK28 antibody; At5g66210 antibody; K2A18.29 antibody; Calcium-dependent protein kinase 28 antibody; EC 2.7.11.1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CPK28 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

May play a role in signal transduction pathways that involve calcium as a second messenger. Acts as developmentally controlled regulator for coordinated stem elongation and vascular development. Acts as key component which contributes to the developmental switch that establishes the transition from vegetative to reproductive growth. Involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling. Interacts with and phosphorylates the kinase BIK1, a central rate-limiting kinase in PTI signaling. Facilitates BIK1 turnover and negatively regulates BIK1-mediated immune responses triggered by several PAMPs. Its kinase activity is necessary and sufficient for its function in PTI signaling.

Gene References into Functions

The work suggests that CPK28 targets MATs (MAT1, MAT2 and MAT3) for degradation by the 26S proteasome pathway, and thus affects ethylene biosynthesis and lignin deposition in Arabidopsis. PMID: 28112445
CPK28 confers developmental processes by the tissue-specific balance of jasmonic acid (JA) and gibberellic acid without affecting JA-mediated defense responses. PMID: 25736059
contributes to the PAMP-induced Ca(2+) burst, supporting its role as a negative regulator of BIK1 PMID: 26039480
CPK28 is a developmentally controlled regulator for coordinated stem elongation and secondary growth. PMID: 23252373

Subcellular Location

Cell membrane; Lipid-anchor.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, CDPK subfamily

Tissue Specificity

Expressed in vascular and meristematic tissues throughout plant development.

Database Links

KEGG: ath:AT5G66210

STRING: 3702.AT5G66210.1

UniGene: At.27527

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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CPK28 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background