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Recombinant Arabidopsis thaliana Calcium-dependent protein kinase 28 (CPK28), partial

Recombinant Arabidopsis thaliana Calcium-dependent protein kinase 28 (CPK28), partial

Code	CSB-YP884278DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP884278DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP884278DOA-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP884278DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP884278DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

CPK28

Uniprot No.

Q9FKW4

Alternative Names

CPK28; At5g66210; K2A18.29; Calcium-dependent protein kinase 28; EC 2.7.11.1

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

May play a role in signal transduction pathways that involve calcium as a second messenger. Acts as developmentally controlled regulator for coordinated stem elongation and vascular development. Acts as key component which contributes to the developmental switch that establishes the transition from vegetative to reproductive growth. Involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling. Interacts with and phosphorylates the kinase BIK1, a central rate-limiting kinase in PTI signaling. Facilitates BIK1 turnover and negatively regulates BIK1-mediated immune responses triggered by several PAMPs. Its kinase activity is necessary and sufficient for its function in PTI signaling.

Gene References into Functions

The work suggests that CPK28 targets MATs (MAT1, MAT2 and MAT3) for degradation by the 26S proteasome pathway, and thus affects ethylene biosynthesis and lignin deposition in Arabidopsis. PMID: 28112445
CPK28 confers developmental processes by the tissue-specific balance of jasmonic acid (JA) and gibberellic acid without affecting JA-mediated defense responses. PMID: 25736059
contributes to the PAMP-induced Ca(2+) burst, supporting its role as a negative regulator of BIK1 PMID: 26039480
CPK28 is a developmentally controlled regulator for coordinated stem elongation and secondary growth. PMID: 23252373

Subcellular Location

Cell membrane; Lipid-anchor.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, CDPK subfamily

Tissue Specificity

Expressed in vascular and meristematic tissues throughout plant development.

Database Links

KEGG: ath:AT5G66210

STRING: 3702.AT5G66210.1

UniGene: At.27527

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