Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CESA6 Polyclonal antibody

Uniprot No.

Q94JQ6

Target Names

CESA6

Alternative Names

CESA6 antibody; IXR2 antibody; PRC1 antibody; QUI antibody; At5g64740 antibody; MVP7.7Cellulose synthase A catalytic subunit 6 [UDP-forming] antibody; AtCesA6 antibody; EC 2.4.1.12 antibody; AraxCelA antibody; Isoxaben-resistant protein 2 antibody; Protein PROCUSTE 1 antibody; Protein QUILL antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CESA6 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation. Involved in the primary cell wall formation. The presence of each protein CESA1 and CESA6 is critical for cell expansion. The hypocotyl elongation is based on a CESA6-dependent cell elongation in dark and a CESA6-independent cell elongation in light. The transition between these two mechanisms requires photosynthesis and PHYB, but not CRY1. The CESA6-dependent cell elongation seems to be independent of gibberellic acid, auxin and ethylene. May be involved in sensitivity to isoxaben. Associates with and moves along cortical microtubules for the process of cellulose deposition.

Gene References into Functions

AtCesA6-null mutants showed a reduced cell elongation of young seedlings with little impact on cell division, which consequently affected cell wall integrity and biomass yield of mature plants. PMID: 29514326
Data indicates that the CC proteins have a broad role in aiding cellulose synthesis during environmental changes and when core complex components are non-functional. [CeSa6] PMID: 26829351
The ordered transverse orientation of microfibrils at the inner face of the cell wall was severely disrupted in prc-1 hypocotyls, particularly in the early growth phase. PMID: 16404578
The Arabidopsis procuste1 (prc1-1) cellulose synthase mutant was studied in order to investigate the role of the cell wall loosening during the early stages of hair formation. PMID: 18485206
PROCUSTE1 has a role in microtubule cortical array organization. PMID: 18583534

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Glycosyltransferase 2 family, Plant cellulose synthase subfamily

Tissue Specificity

Expressed in germinating seeds, seedlings, roots, stems, leaves and flowers. Not present in mature flowers.

Database Links

KEGG: ath:AT5G64740

STRING: 3702.AT5G64740.1

UniGene: At.9777

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CESA6 Antibody

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