Recombinant Arabidopsis thaliana Cellulose synthase A catalytic subunit 6 [UDP-forming] (CESA6), partial

Code CSB-YP853234DOA
MSDS
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Source Yeast
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Code CSB-EP853234DOA
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Source E.coli
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Code CSB-EP853234DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP853234DOA
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Source Baculovirus
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Code CSB-MP853234DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CESA6
Uniprot No.
Alternative Names
CESA6; IXR2; PRC1; QUI; At5g64740; MVP7.7Cellulose synthase A catalytic subunit 6 [UDP-forming]; AtCesA6; EC 2.4.1.12; AraxCelA; Isoxaben-resistant protein 2; Protein PROCUSTE 1; Protein QUILL
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation. Involved in the primary cell wall formation. The presence of each protein CESA1 and CESA6 is critical for cell expansion. The hypocotyl elongation is based on a CESA6-dependent cell elongation in dark and a CESA6-independent cell elongation in light. The transition between these two mechanisms requires photosynthesis and PHYB, but not CRY1. The CESA6-dependent cell elongation seems to be independent of gibberellic acid, auxin and ethylene. May be involved in sensitivity to isoxaben. Associates with and moves along cortical microtubules for the process of cellulose deposition.
Gene References into Functions
  1. AtCesA6-null mutants showed a reduced cell elongation of young seedlings with little impact on cell division, which consequently affected cell wall integrity and biomass yield of mature plants. PMID: 29514326
  2. Data indicates that the CC proteins have a broad role in aiding cellulose synthesis during environmental changes and when core complex components are non-functional. [CeSa6] PMID: 26829351
  3. The ordered transverse orientation of microfibrils at the inner face of the cell wall was severely disrupted in prc-1 hypocotyls, particularly in the early growth phase. PMID: 16404578
  4. The Arabidopsis procuste1 (prc1-1) cellulose synthase mutant was studied in order to investigate the role of the cell wall loosening during the early stages of hair formation. PMID: 18485206
  5. PROCUSTE1 has a role in microtubule cortical array organization. PMID: 18583534

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Glycosyltransferase 2 family, Plant cellulose synthase subfamily
Tissue Specificity
Expressed in germinating seeds, seedlings, roots, stems, leaves and flowers. Not present in mature flowers.
Database Links

KEGG: ath:AT5G64740

STRING: 3702.AT5G64740.1

UniGene: At.9777

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