cheW Antibody

Code CSB-PA364282XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) cheW Polyclonal antibody
Uniprot No.
Target Names
cheW
Alternative Names
cheW antibody; b1887 antibody; JW1876Chemotaxis protein CheW antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) cheW protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. It physically bridges CheA to the MCPs (methyl-accepting chemotaxis proteins) to allow regulated phosphotransfer to CheY and CheB.
Gene References into Functions
  1. Results suggest that ligand binding triggers changes in the receptor that alter its signalling contacts with CheW. PMID: 25060668
  2. Co-overexpression of Tsr, CheA, and CheW yields hexagonal Arrays. PMID: 24580139
  3. analysis of CheA and CheW protein architecture and their role in bacterial chemotaxis PMID: 22355139
  4. The exchange of CheA and CheW at receptor clusters shows a modest dependency on the receptor modification. PMID: 21978288
  5. Results identified CheW surfaces responsible for CheA-Tsr interaction. PMID: 21463637
  6. The 2[to]2[to]1 organization is the core structural and functional unit of chemotaxis signaling complexes and hexagonal arrays characteristic of signaling complexes may be built from this unit. PMID: 21606342
  7. The authors propose that the CheW-binding sites in receptor dimers overlap their trimer contact sites and that high levels of CheW saturate the receptor-binding sites, preventing trimer assembly. PMID: 20487303
  8. The synergistic effect of serine and CheW binding to Tsr is attributed to distinct influences on receptor structure PMID: 15766389
  9. chemotaxis in E. coli requires the presence of chemoreceptor arrays and that the formation of these arrays requires the scaffolding interactions of the signaling molecules CheA and CheW PMID: 17360429

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Subcellular Location
Cytoplasm.
Database Links
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