cheZ Antibody

^{Rare Species}

Datasheet

Code	CSB-PA364334XA01ENV
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: cheZ Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31 kDa Observed band size: 31 kDa
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) cheZ Polyclonal antibody

Uniprot No.

P0A9H9

Target Names

cheZ

Alternative Names

Protein phosphatase CheZ (EC 3.1.3.-) (Chemotaxis protein CheZ) cheZ b1881 JW1870

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) cheZ protein (1-214aa)

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).

Gene References into Functions

The suitability for FRET analysis as well as functionality of fusions with SNAP- and HaloTag could be demonstrated by studying the transient interaction between chemotaxis response regulator CheY and its phosphatase CheZ PMID: 25856398
Amino acid substitutions distant from the active site can affect CheZ catalytic activity and CheYp binding, perhaps via the propagation of structural or dynamic perturbations through a helical bundle. PMID: 21764922
simulations suggest that dynamic CheZ localization will sharpen cellular responses to chemoeffectors, increase the range of detectable ligand concentrations, and make adaptation more precise and robust PMID: 16683020
Specific CheA-short (CheA(S)) residues, L123 and L126, were identified as critical for CheZ binding. PMID: 19581362

Subcellular Location

Cytoplasm. Note=Colocalizes with CheA chemoreceptor patches near the cell poles, which requires CheA(short).

Protein Families

CheZ family

Database Links

KEGG: ecj:JW1870

STRING: 316385.ECDH10B_2022

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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cheZ Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background