CHLG Antibody

Code CSB-PA656431XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CHLG Polyclonal antibody
Uniprot No.
Target Names
CHLG
Alternative Names
CHLG; G4; At3g51820; ATEM1.7; Chlorophyll synthase, chloroplastic; Polyprenyl transferase; Protein G4; AtG4
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CHLG protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in one of the last steps of the biosynthesis of chlorophyll a. Catalyzes the esterification of chlorophillide a or b with a preference for geranylgeranyldiphosphate (GGPP) rather than for phytyldiphosphate (PhyPP).
Gene References into Functions
  1. Data show that leaves of RNA interference lines with partial suppression of chloriphyll synthase (CHLSYN; At3g51820) CHLSYN displayed marked reductions in chlorophyll but up to a 2-fold increase in tocopherol concentrations. PMID: 26048882
  2. Chlorophyll synthase acts in a salvage pathway for chlorophyll biosynthesis by re-esterifying the chlorophyllide a produced during chlorophyll turnover. PMID: 25041167
  3. AtAtg4a is the predominant protease and it processes AtAtg8a, AtAtg8c, AtAtg8d, and AtAtg8i better than AtAtg4b in vitro. PMID: 24379391
Subcellular Location
Plastid, chloroplast membrane; Multi-pass membrane protein.
Protein Families
UbiA prenyltransferase family, Chlorophyll synthase subfamily
Tissue Specificity
Low level in flower buds, flowers, stems, leaves, greening cotyledons and immature siliques, but not in mature siliques or seeds.
Database Links

KEGG: ath:AT3G51820

STRING: 3702.AT3G51820.1

UniGene: At.5423

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