Recombinant Arabidopsis thaliana Chlorophyll synthase, chloroplastic (CHLG)

Code CSB-CF656431DOA
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
CHLG
Uniprot No.
Alternative Names
CHLG; G4; At3g51820; ATEM1.7; Chlorophyll synthase, chloroplastic; Polyprenyl transferase; Protein G4; AtG4
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
58-387
Target Protein Sequence
AAETDTDKVKSQTPDKAPAGGSSINQLLGIKGASQETNKWKIRLQLTKPVTWPPLVWGVV CGAAASGNFHWTPEDVAKSILCMMMSGPCLTGYTQTINDWYDRDIDAINEPYRPIPSGAI SEPEVITQVWVLLLGGLGIAGILDVWAGHTTPTVFYLALGGSLLSYIYSAPPLKLKQNGW VGNFALGASYISLPWWAGQALFGTLTPDVVVLTLLYSIAGLGIAIVNDFKSVEGDRALGL QSLPVAFGTETAKWICVGAIDITQLSVAGYLLASGKPYYALALVALIIPQIVFQFKYFLK DPVKYDVKYQASAQPFLVLGIFVTALASQH
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in one of the last steps of the biosynthesis of chlorophyll a. Catalyzes the esterification of chlorophillide a or b with a preference for geranylgeranyldiphosphate (GGPP) rather than for phytyldiphosphate (PhyPP).
Gene References into Functions
  1. Data show that leaves of RNA interference lines with partial suppression of chloriphyll synthase (CHLSYN; At3g51820) CHLSYN displayed marked reductions in chlorophyll but up to a 2-fold increase in tocopherol concentrations. PMID: 26048882
  2. Chlorophyll synthase acts in a salvage pathway for chlorophyll biosynthesis by re-esterifying the chlorophyllide a produced during chlorophyll turnover. PMID: 25041167
  3. AtAtg4a is the predominant protease and it processes AtAtg8a, AtAtg8c, AtAtg8d, and AtAtg8i better than AtAtg4b in vitro. PMID: 24379391
Subcellular Location
Plastid, chloroplast membrane; Multi-pass membrane protein.
Protein Families
UbiA prenyltransferase family, Chlorophyll synthase subfamily
Tissue Specificity
Low level in flower buds, flowers, stems, leaves, greening cotyledons and immature siliques, but not in mature siliques or seeds.
Database Links

KEGG: ath:AT3G51820

STRING: 3702.AT3G51820.1

UniGene: At.5423

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