CHUP1 Antibody

Code CSB-PA885350XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CHUP1 Polyclonal antibody
Uniprot No.
Target Names
CHUP1
Alternative Names
CHUP1 antibody; At3g25690 antibody; T5M7.14Protein CHUP1 antibody; chloroplastic antibody; Protein CHLOROPLAST UNUSUAL POSITIONING 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CHUP1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for the positioning and movement of chloroplasts. Interacts with profilin and actin independent of its polymerization status. Regulates chloroplast localization by anchoring chloroplasts to the plasma membrane and forming a bridge to the actin cytoskeleton.
Gene References into Functions
  1. The results indicate that CHUP1 and KAC1/KAC2 function somewhat independently, but interdependently mediate both chloroplast and nuclear photorelocation movements in Arabidopsis cells. PMID: 27310016
  2. CHUP1 functions as a dimer and the leucine zippers in the N-terminal and C-terminal portion of the protein are involved in intramolecular interactions. PMID: 21421414
  3. After 5 minutes of exposure to blue light, there were significant differences in expression patterns in phot1 and phot2 mutants from that of the chup1 mutant. PMID: 21309982
  4. Under high light conditions, chup1 plants assumed significantly different guard cell chloroplast positions on both leaf surfaces than WT plants. PMID: 20614182
  5. Data show that CHUP1 as a linker between chloroplasts and the cytoskeleton, by interacting with actin and profilin. PMID: 18193273
  6. CHUP1 localizes on the outer envelope of chloroplasts and anchors chloroplasts to the plasma membrane, forming a bridge to the actin cytoskeleton. PMID: 18715957

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Subcellular Location
Plastid, chloroplast outer membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue Specificity
Expressed in cauline leaves, rosette leaves, stems and flowers, but not in roots.
Database Links

KEGG: ath:AT3G25690

STRING: 3702.AT3G25690.1

UniGene: At.27741

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