Recombinant Arabidopsis thaliana Protein CHUP1, chloroplastic (CHUP1), partial

Code CSB-YP885350DOA
MSDS
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Source Yeast
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Code CSB-EP885350DOA
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Source E.coli
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Code CSB-EP885350DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP885350DOA
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Source Baculovirus
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Code CSB-MP885350DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CHUP1
Uniprot No.
Alternative Names
CHUP1; At3g25690; T5M7.14Protein CHUP1; chloroplastic; Protein CHLOROPLAST UNUSUAL POSITIONING 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for the positioning and movement of chloroplasts. Interacts with profilin and actin independent of its polymerization status. Regulates chloroplast localization by anchoring chloroplasts to the plasma membrane and forming a bridge to the actin cytoskeleton.
Gene References into Functions
  1. The results indicate that CHUP1 and KAC1/KAC2 function somewhat independently, but interdependently mediate both chloroplast and nuclear photorelocation movements in Arabidopsis cells. PMID: 27310016
  2. CHUP1 functions as a dimer and the leucine zippers in the N-terminal and C-terminal portion of the protein are involved in intramolecular interactions. PMID: 21421414
  3. After 5 minutes of exposure to blue light, there were significant differences in expression patterns in phot1 and phot2 mutants from that of the chup1 mutant. PMID: 21309982
  4. Under high light conditions, chup1 plants assumed significantly different guard cell chloroplast positions on both leaf surfaces than WT plants. PMID: 20614182
  5. Data show that CHUP1 as a linker between chloroplasts and the cytoskeleton, by interacting with actin and profilin. PMID: 18193273
  6. CHUP1 localizes on the outer envelope of chloroplasts and anchors chloroplasts to the plasma membrane, forming a bridge to the actin cytoskeleton. PMID: 18715957

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Subcellular Location
Plastid, chloroplast outer membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue Specificity
Expressed in cauline leaves, rosette leaves, stems and flowers, but not in roots.
Database Links

KEGG: ath:AT3G25690

STRING: 3702.AT3G25690.1

UniGene: At.27741

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