Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans clh-3 Polyclonal antibody

Uniprot No.

Q9BMK9

Target Names

clh-3

Alternative Names

clh-3 antibody; clc-3 antibody; E04F6.11Chloride channel protein clh-3 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans clh-3 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Voltage-gated chloride channel. Insensitive to depolarizing conditioning voltages, requires low voltages for activation, insensitive to chloride levels and has a mild sensitivity to low pH. Channel gating properties are conferred by the cytoplasmic C-terminus. Plays a role in egg laying by modulating hermaphrodite-specific neurons (HSN) excitability and the ovulatory contractions of gap-junction-coupled gonadal sheath cells. When active, may prevent tubular formation of the excretory canals. Activated during oocyte meiotic maturation and by membrane hyperpolarization and cell swelling. Inhibited by Zn(2+) and to a lesser extent by Cd(2+)(PubMed:11231150).; Voltage-gated chloride channel. Sensitive to depolarizing conditioning voltages, requires stronger voltages for activation and activation is slower, is inhibited by low concentrations of chloride and is activated by low pH. Channel gating properties are conferred by the cytoplasmic C-terminus.

Gene References into Functions

Channels encoded by the CLC-2 homolog clh-3 regulate the activity of neurons which control egg-laying behavior. PMID: 24431435
Postulate that the R-helix linker interacts with CBS2 alpha2, and that this putative interaction provides a pathway by which cytoplasmic C-terminus conformational changes induce conformational changes in membrane domains that in turn modulate CLH-3 function. PMID: 20581474
Alternative splicing of CLH-3 amino- and carboxyl-termini modifies the functional properties of the channel by altering the accessibility and/or function of pore-associated ion-binding sites. PMID: 14565992
These results suggest that the deleted COOH-terminal region of CLH-3bDeltaC functions to modulate channel sensitivity to voltage and extracellular ions. PMID: 16306126

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Chloride channel (TC 2.A.49) family

Tissue Specificity

Expressed in excretory cell, 4 anterior epithelial cells of the intestine, hermaphrodite-specific neurons and enteric muscles. Expressed also in vulva and uterus. Isoform a is expressed in oocytes (at protein level).

Database Links

STRING: 6239.E04F6.11a

UniGene: Cel.19436

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clh-3 Antibody

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