Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CLPC2 Polyclonal antibody

Uniprot No.

Q9SXJ7

Target Names

CLPC2

Alternative Names

CLPC2 antibody; HSP93-III antibody; At3g48870 antibody; T21J18.140 antibody; Chaperone protein ClpC2 antibody; chloroplastic antibody; EC 3.6.1.3 antibody; ATP-dependent Clp protease ATP-binding subunit ClpC homolog 2 antibody; AtClpC antibody; Casein lytic proteinase C2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CLPC2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Molecular chaperone. May act as a suppressor of FtsH-mediated thylakoid membrane biogenesis and may enhance photoinhibition. Seems not involved in chloroplastic protein import. Probable component of the TIC-associated stromal import motor involved in inner membrane translocation. Has an ATPase activity, but no ADPase activity. Interacts with transit peptides with a positional preference. Localization of the signal sequence at the N-terminal end of a protein seems mandatory for interaction to take place.

Gene References into Functions

AtClpS1 allows for high-affinity binding to classical N-degrons of the bacterial N-end rule. PMID: 29401302
the proteolytic activity of Clp protease counteracts GBP binding to assure the appropriate content of GluTR and the adequate amino levulinic acid synthesis for chlorophyll and heme in higher plants. PMID: 26884485
Both AtClpC2 and AtClpD interacted with the transit peptides when they were located at the N-terminal position of a protein, but not when they were allocated to the C-terminal end or at the interior of a polypeptide. [ClpC2] PMID: 22545953
The association of Hsp93 with the inner envelope membrane through its amino-terminal domain is important for the functions of Hsp93 in vivo.[Hsp93] PMID: 22353577
Data show that ClpS1 (AT1G68660) interacts with plastid ClpC1 (AT5G50920), ClpC2 (AT3G48870), but not with ClpT1 (AT4G25370) or ClpT2 (AT4G12060). PMID: 23898032
The interaction of Tic110, Tic40 and Hsp93 in A. thaliana chloroplast protein transport is described. [Hsp93] PMID: 15659100

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Subcellular Location

Plastid, chloroplast stroma. Plastid, chloroplast membrane.

Protein Families

ClpA/ClpB family, ClpC subfamily

Tissue Specificity

Expressed at low levels in roots and inflorescences. Expressed at very low levels in rosette leaves. Expressed in photosynthetic green tissues with high levels in young, developing leaf tissues.

Database Links

KEGG: ath:AT3G48870

STRING: 3702.AT3G48870.1

UniGene: At.23421

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