Recombinant Arabidopsis thaliana Chaperone protein ClpC2, chloroplastic (CLPC2), partial

Code CSB-YP874552DOA
MSDS
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Source Yeast
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Code CSB-EP874552DOA
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Source E.coli
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Code CSB-EP874552DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874552DOA
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Source Baculovirus
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Code CSB-MP874552DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CLPC2
Uniprot No.
Alternative Names
CLPC2; HSP93-III; At3g48870; T21J18.140; Chaperone protein ClpC2; chloroplastic; EC 3.6.1.3; ATP-dependent Clp protease ATP-binding subunit ClpC homolog 2; AtClpC; Casein lytic proteinase C2
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Molecular chaperone. May act as a suppressor of FtsH-mediated thylakoid membrane biogenesis and may enhance photoinhibition. Seems not involved in chloroplastic protein import. Probable component of the TIC-associated stromal import motor involved in inner membrane translocation. Has an ATPase activity, but no ADPase activity. Interacts with transit peptides with a positional preference. Localization of the signal sequence at the N-terminal end of a protein seems mandatory for interaction to take place.
Gene References into Functions
  1. AtClpS1 allows for high-affinity binding to classical N-degrons of the bacterial N-end rule. PMID: 29401302
  2. the proteolytic activity of Clp protease counteracts GBP binding to assure the appropriate content of GluTR and the adequate amino levulinic acid synthesis for chlorophyll and heme in higher plants. PMID: 26884485
  3. Both AtClpC2 and AtClpD interacted with the transit peptides when they were located at the N-terminal position of a protein, but not when they were allocated to the C-terminal end or at the interior of a polypeptide. [ClpC2] PMID: 22545953
  4. The association of Hsp93 with the inner envelope membrane through its amino-terminal domain is important for the functions of Hsp93 in vivo.[Hsp93] PMID: 22353577
  5. Data show that ClpS1 (AT1G68660) interacts with plastid ClpC1 (AT5G50920), ClpC2 (AT3G48870), but not with ClpT1 (AT4G25370) or ClpT2 (AT4G12060). PMID: 23898032
  6. The interaction of Tic110, Tic40 and Hsp93 in A. thaliana chloroplast protein transport is described. [Hsp93] PMID: 15659100

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Subcellular Location
Plastid, chloroplast stroma. Plastid, chloroplast membrane.
Protein Families
ClpA/ClpB family, ClpC subfamily
Tissue Specificity
Expressed at low levels in roots and inflorescences. Expressed at very low levels in rosette leaves. Expressed in photosynthetic green tissues with high levels in young, developing leaf tissues.
Database Links

KEGG: ath:AT3G48870

STRING: 3702.AT3G48870.1

UniGene: At.23421

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