CMT3 Antibody

Datasheet

Code	CSB-PA839126XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CMT3 Polyclonal antibody

Uniprot No.

Q94F88

Target Names

CMT3

Alternative Names

CMT3 antibody; At1g69770 antibody; T6C23.3 antibody; DNA antibody; cytosine-5)-methyltransferase CMT3 antibody; EC 2.1.1.37 antibody; Chromomethylase 3 antibody; Protein CHROMOMETHYLASE 3 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CMT3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Involved in the CpXpG methylation and in gene silencing. Methylates preferentially transposon-related sequences. Functionally redundant to DRM1/DRM2 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation.

Gene References into Functions

Cmt3gene expression throughout normal development in Arabidopsis PMID: 25842863
gamma irradiation induced DNA hypomethylation preferentially at non-CG sites of transcriptionally inactive repeats in a locus-specific manner, which depends on CMT3 activity PMID: 24279389
These results suggest that CMT3 associates with H3K9me2-containing nucleosomes through dual binding of its bromo adjacent homology and chromo domains to H3K9me2 in order to target DNA methylation. PMID: 23021223
A speculative model of the CMT3-induced egg cell silencing is presented here. PMID: 20505370
Results suggest a model in which methylation of histone H3 lysines 9 and 27 provide a combinatorial histone code for the recruitment of CHROMOMETHYLASE3 to silent loci. PMID: 15457214

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Subcellular Location

Nucleus.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, C5-methyltransferase family

Database Links

KEGG: ath:AT1G69770

STRING: 3702.AT1G69770.1

UniGene: At.35376

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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CMT3 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background