Recombinant Arabidopsis thaliana DNA (cytosine-5)-methyltransferase CMT3 (CMT3), partial

Code CSB-YP839126DOA
MSDS
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Source Yeast
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Code CSB-EP839126DOA
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Source E.coli
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Code CSB-EP839126DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839126DOA
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Source Baculovirus
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Code CSB-MP839126DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CMT3
Uniprot No.
Alternative Names
CMT3; At1g69770; T6C23.3; DNA; cytosine-5)-methyltransferase CMT3; EC 2.1.1.37; Chromomethylase 3; Protein CHROMOMETHYLASE 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the CpXpG methylation and in gene silencing. Methylates preferentially transposon-related sequences. Functionally redundant to DRM1/DRM2 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation.
Gene References into Functions
  1. Cmt3gene expression throughout normal development in Arabidopsis PMID: 25842863
  2. gamma irradiation induced DNA hypomethylation preferentially at non-CG sites of transcriptionally inactive repeats in a locus-specific manner, which depends on CMT3 activity PMID: 24279389
  3. These results suggest that CMT3 associates with H3K9me2-containing nucleosomes through dual binding of its bromo adjacent homology and chromo domains to H3K9me2 in order to target DNA methylation. PMID: 23021223
  4. A speculative model of the CMT3-induced egg cell silencing is presented here. PMID: 20505370
  5. Results suggest a model in which methylation of histone H3 lysines 9 and 27 provide a combinatorial histone code for the recruitment of CHROMOMETHYLASE3 to silent loci. PMID: 15457214

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Subcellular Location
Nucleus.
Protein Families
Class I-like SAM-binding methyltransferase superfamily, C5-methyltransferase family
Database Links

KEGG: ath:AT1G69770

STRING: 3702.AT1G69770.1

UniGene: At.35376

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