Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CAMTA3 Polyclonal antibody

Uniprot No.

Q8GSA7

Target Names

CAMTA3

Alternative Names

CAMTA3 antibody; CMTA3 antibody; SARD3 antibody; SR1 antibody; At2g22300 antibody; T26C19.4Calmodulin-binding transcription activator 3 antibody; AtCAMTA3 antibody; Ethylene-induced calmodulin-binding protein 1 antibody; EICBP1 antibody; Ethylene-induced calmodulin-binding protein a antibody; EICBP.a antibody; Protein SAR-DEFICIENT 3 antibody; Signal-responsive protein 1 antibody; AtSR1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CAMTA3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription activator that binds to the DNA consensus sequence 5'-[ACG]CGCG[GTC]-3'. Binds calmodulin in a calcium-dependent manner in vitro. Regulates transcriptional activity in response to calcium signals. Involved in freezing tolerance in association with CAMTA1 and CAMTA2. Required for the cold-induced expression of DREB1B/CBF1, DREB1C/CBF2, ZAT12 and GOLS3. Involved in response to cold. Contributes together with CAMTA5 to the positive regulation of the cold-induced expression of DREB1A/CBF3, DREB1B/CBF1 and DREB1C/CBF2. Involved together with CAMTA2 and CAMTA4 in the positive regulation of a general stress response (GSR). Involved in the regulation of GSR amplitude downstream of MEKK1. Involved in the regulation of a set of genes involved in defense responses against pathogens. Involved in the regulation of both basal resistance and systemic acquired resistance (SAR). Acts as negative regulator of plant immunity. Binds to the promoter of the defense-related gene EDS1 and represses its expression. Binds to the promoter of the defense-related gene NDR1 and represses its expression. Involved in defense against insects. Required for tolerance to the generalist herbivore Trichoplusia ni, and contributes to the positive regulation of genes associated with glucosinolate metabolism. Required for tolerance to Bradysia impatiens larvae. Mediates herbivore-induced wound response. Required for wound-induced jasmonate accumulation. Involved in the regulation of ethylene-induced senescence by binding to the promoter of the senescence-inducer gene EIN3 and repressing its expression.

Gene References into Functions

Genetics and pharmacological approaches confirmed the specificity of MEcPP in rapid stress response element (RSRE) induction via the transcription factor CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 3 (CAMTA3), in a calcium-dependent manner. PMID: 27432993
These findings support a model in which DSC1 and DSC2 guard CAMTA3, and they suggest that other negative regulators of immunity may similarly represent guardees. PMID: 28407487
double mekk1/camta3 mutant positioned CAMTA3 downstream of MEKK1 and verified their distinct roles in GSR regulation. mekk1-5 displays programmed cell death and overaccumulates reactive oxygen species and salicylic acid PMID: 25157030
CAMTA1, CAMTA2 and CAMTA3 function together to inhibit salicylic acid biosynthesis at warm temperature (22 degrees C).CAMTA1, CAMTA2 and CAMTA3 function together to positively regulate CBF1, CBF2 and CBF3 and freezing tolerance. PMID: 23581962
SR1 plays an important role in plant immunity and ethylene signaling by directly regulating NDR1 and EIN3. PMID: 22345509
Ca(2+)/Calmodulin-mediated signaling regulates plant response to herbivore attack/wounding by modulating the salicylic acid-jasmonic acid crosstalk through AtSR1. PMID: 22371088
CAMTA3 regulates the expression of a set of genes involved in biotic defense responses. PMID: 18298954

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Subcellular Location

Nucleus.

Protein Families

CAMTA family

Tissue Specificity

Expressed in roots, stems, leaves, carpels, and siliques, but not in stigmas or other parts of the flower.

Database Links

KEGG: ath:AT2G22300

STRING: 3702.AT2G22300.1

UniGene: At.48505

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CAMTA3 Antibody

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