Recombinant Arabidopsis thaliana Calmodulin-binding transcription activator 3 (CMTA3), partial

Code CSB-YP808401DOA
MSDS
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Source Yeast
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Code CSB-EP808401DOA
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Source E.coli
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Code CSB-EP808401DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP808401DOA
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Source Baculovirus
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Code CSB-MP808401DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CAMTA3
Uniprot No.
Alternative Names
CAMTA3; CMTA3; SARD3; SR1; At2g22300; T26C19.4Calmodulin-binding transcription activator 3; AtCAMTA3; Ethylene-induced calmodulin-binding protein 1; EICBP1; Ethylene-induced calmodulin-binding protein a; EICBP.a; Protein SAR-DEFICIENT 3; Signal-responsive protein 1; AtSR1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription activator that binds to the DNA consensus sequence 5'-[ACG]CGCG[GTC]-3'. Binds calmodulin in a calcium-dependent manner in vitro. Regulates transcriptional activity in response to calcium signals. Involved in freezing tolerance in association with CAMTA1 and CAMTA2. Required for the cold-induced expression of DREB1B/CBF1, DREB1C/CBF2, ZAT12 and GOLS3. Involved in response to cold. Contributes together with CAMTA5 to the positive regulation of the cold-induced expression of DREB1A/CBF3, DREB1B/CBF1 and DREB1C/CBF2. Involved together with CAMTA2 and CAMTA4 in the positive regulation of a general stress response (GSR). Involved in the regulation of GSR amplitude downstream of MEKK1. Involved in the regulation of a set of genes involved in defense responses against pathogens. Involved in the regulation of both basal resistance and systemic acquired resistance (SAR). Acts as negative regulator of plant immunity. Binds to the promoter of the defense-related gene EDS1 and represses its expression. Binds to the promoter of the defense-related gene NDR1 and represses its expression. Involved in defense against insects. Required for tolerance to the generalist herbivore Trichoplusia ni, and contributes to the positive regulation of genes associated with glucosinolate metabolism. Required for tolerance to Bradysia impatiens larvae. Mediates herbivore-induced wound response. Required for wound-induced jasmonate accumulation. Involved in the regulation of ethylene-induced senescence by binding to the promoter of the senescence-inducer gene EIN3 and repressing its expression.
Gene References into Functions
  1. Genetics and pharmacological approaches confirmed the specificity of MEcPP in rapid stress response element (RSRE) induction via the transcription factor CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 3 (CAMTA3), in a calcium-dependent manner. PMID: 27432993
  2. These findings support a model in which DSC1 and DSC2 guard CAMTA3, and they suggest that other negative regulators of immunity may similarly represent guardees. PMID: 28407487
  3. double mekk1/camta3 mutant positioned CAMTA3 downstream of MEKK1 and verified their distinct roles in GSR regulation. mekk1-5 displays programmed cell death and overaccumulates reactive oxygen species and salicylic acid PMID: 25157030
  4. CAMTA1, CAMTA2 and CAMTA3 function together to inhibit salicylic acid biosynthesis at warm temperature (22 degrees C).CAMTA1, CAMTA2 and CAMTA3 function together to positively regulate CBF1, CBF2 and CBF3 and freezing tolerance. PMID: 23581962
  5. SR1 plays an important role in plant immunity and ethylene signaling by directly regulating NDR1 and EIN3. PMID: 22345509
  6. Ca(2+)/Calmodulin-mediated signaling regulates plant response to herbivore attack/wounding by modulating the salicylic acid-jasmonic acid crosstalk through AtSR1. PMID: 22371088
  7. CAMTA3 regulates the expression of a set of genes involved in biotic defense responses. PMID: 18298954

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Subcellular Location
Nucleus.
Protein Families
CAMTA family
Tissue Specificity
Expressed in roots, stems, leaves, carpels, and siliques, but not in stigmas or other parts of the flower.
Database Links

KEGG: ath:AT2G22300

STRING: 3702.AT2G22300.1

UniGene: At.48505

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