CBL3 Antibody

Datasheet

Code	CSB-PA818587XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CBL3 Polyclonal antibody

Uniprot No.

Q8LEM7

Target Names

CBL3

Alternative Names

CBL3 antibody; SCABP6 antibody; At4g26570 antibody; T15N24.20Calcineurin B-like protein 3 antibody; SOS3-like calcium-binding protein 6 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CBL3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binds calcium ions. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Negatively regulates the enzyme activity of MTN1 in the presence of calcium.

Gene References into Functions

AtMTAN2 associated exclusively with CBL3. PMID: 26259190
crucial regulator of vacuolar dynamics and polarized pollen tube growth PMID: 25936548
While single cbl2 or cbl3 mutants did not display discernable phenotypes, cbl2/cbl3 mutants exhibited defects in vegetative growth and were severely impaired in seed development and morphology. PMID: 24479654
CBL3 and CIPK9 regulates potassium homeostasis under low potassium stress. PMID: 23109687
The tonoplast-localized CBL3 is critical for plant development and ion homeostasis in plant cells, at least in part through regulation of vacuolar H+-ATPase activity in plants. PMID: 23184060
CBL3 and ATMTN1 interact only in the presence of Ca(2+). PMID: 18945934
CBL3-GFP exhibited dynamic patterns of subcellular localization, including several endomembrane compartments, the apical plasma membrane (PM), and cytoskeleton-like structures in pollen tubes. PMID: 19686372

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Subcellular Location

Vacuole membrane; Lipid-anchor. Note=Tonoplast localization abolished by 2-bromopalmitate (2-BP) treatment.

Protein Families

Calcineurin regulatory subunit family

Tissue Specificity

Ubiquitous. Stronger expression in roots. Expressed in root tip and root hair zone, leaf veins, vascular bundles and vasculature of sepals.

Database Links

KEGG: ath:AT4G26570

STRING: 3702.AT4G26570.2

UniGene: At.22453

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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CBL3 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background