Recombinant Arabidopsis thaliana Calcineurin B-like protein 3 (CBL3)

Code CSB-YP818587DOA
MSDS
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Source Yeast
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Code CSB-EP818587DOA
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Source E.coli
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Code CSB-EP818587DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP818587DOA
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Source Baculovirus
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Code CSB-MP818587DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CBL3
Uniprot No.
Alternative Names
CBL3; SCABP6; At4g26570; T15N24.20Calcineurin B-like protein 3; SOS3-like calcium-binding protein 6
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-226
Target Protein Sequence
MSQCIDGFKH VCSSFFRCFD IDIYKQSGGL GDPELLARET VFSVSEIEAL YELFKKISSA VIDDGLINKE EFQLALFKTN KKESLFADRV FDLFDTKHNG ILGFEEFARA LSVFHPNAPI EDKIDFSFQL YDLKQQGFIE RQEVKQMVVA TLAESGMNLS DEIIESIIDK TFEEADTKHD GRIDKEEWRT LVLRHPSLLK NMTLQYLKDI TTTFPSFVFH SQVEDT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binds calcium ions. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Negatively regulates the enzyme activity of MTN1 in the presence of calcium.
Gene References into Functions
  1. AtMTAN2 associated exclusively with CBL3. PMID: 26259190
  2. crucial regulator of vacuolar dynamics and polarized pollen tube growth PMID: 25936548
  3. While single cbl2 or cbl3 mutants did not display discernable phenotypes, cbl2/cbl3 mutants exhibited defects in vegetative growth and were severely impaired in seed development and morphology. PMID: 24479654
  4. CBL3 and CIPK9 regulates potassium homeostasis under low potassium stress. PMID: 23109687
  5. The tonoplast-localized CBL3 is critical for plant development and ion homeostasis in plant cells, at least in part through regulation of vacuolar H+-ATPase activity in plants. PMID: 23184060
  6. CBL3 and ATMTN1 interact only in the presence of Ca(2+). PMID: 18945934
  7. CBL3-GFP exhibited dynamic patterns of subcellular localization, including several endomembrane compartments, the apical plasma membrane (PM), and cytoskeleton-like structures in pollen tubes. PMID: 19686372

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Subcellular Location
Vacuole membrane; Lipid-anchor. Note=Tonoplast localization abolished by 2-bromopalmitate (2-BP) treatment.
Protein Families
Calcineurin regulatory subunit family
Tissue Specificity
Ubiquitous. Stronger expression in roots. Expressed in root tip and root hair zone, leaf veins, vascular bundles and vasculature of sepals.
Database Links

KEGG: ath:AT4G26570

STRING: 3702.AT4G26570.2

UniGene: At.22453

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