Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CBL10 Polyclonal antibody

Uniprot No.

Q7FRS8

Target Names

CBL10

Alternative Names

CBL10 antibody; At4g33000 antibody; F26P21_120Calcineurin B-like protein 10 antibody; SOS3-like calcium binding protein 8 antibody; SCaBP8 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CBL10 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Mediates salt tolerance, but only when phosphorylated. Competes with CIPK23 for a direct binding to AKT1, negatively regulating its activity via a protein kinase-independent mechanism.

Gene References into Functions

CBL10 is critical for reproductive development in the presence of salt and that it functions in different pathways during vegetative and reproductive development. PMID: 26979332
CBL10 negatively affects tolerance to drought and is not mediated by abscisic acid-induced signaling. Ca(2+) signaling through CBL10 differently affects the response to abiotic stresses, partly by regulating BR sensitivity of plant tissues. PMID: 26795150
mutation of SCABP8 suppresses the ant-knockout salt-tolerant phenotype, implying that ANT functions as a negative transcriptional regulator of SCABP8 upon salt stress. PMID: 26054800
The study revealed a CBL-interacting protein kinase-independent regulatory mechanism of calcineurin B-like proteins in which CBL10 directly regulates AKT1 activity and affects ion homeostasis in plant cells. PMID: 23331977
SCABP8 and SOS3 are only partially redundant in their function, and each plays additional and unique roles in the plant salt stress response. PMID: 17449811
findings suggest that CBL10 and CIPK24 (SOS2) constitute a novel salt-tolerance pathway that regulates the sequestration/compartmentalization of Na(+) in plant cells PMID: 17825054
SCaBP8 phosphorylation is required for the activation of SOS1 and has a role in regulating salt tolerance in Arabidopsis. PMID: 19448033

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Subcellular Location

Vacuole membrane. Endosome. Cell membrane.

Protein Families

Calcineurin regulatory subunit family

Tissue Specificity

Expressed in shoots, leaves, stems, flowers and siliques. Barely detected in roots.

Database Links

KEGG: ath:AT4G33000

STRING: 3702.AT4G33000.1

UniGene: At.31618

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CBL10 Antibody

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