Recombinant Arabidopsis thaliana Calcineurin B-like protein 10 (CBL10)

Code CSB-YP762517DOA
MSDS
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Source Yeast
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Code CSB-EP762517DOA
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Source E.coli
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Code CSB-EP762517DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP762517DOA
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Source Baculovirus
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Code CSB-MP762517DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CBL10
Uniprot No.
Alternative Names
CBL10; At4g33000; F26P21_120Calcineurin B-like protein 10; SOS3-like calcium binding protein 8; SCaBP8
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-256
Target Protein Sequence
MTTGRPNNIL ALKISTRSSS LTVGEQFCAV FIPFFAIIDV LVSSVGQCFD CRSTSPRTCQ HADLERLARE SQFSVNEVEA LYELFKKLSC SIIDDGLIHK EELRLALFQA PYGENLFLDR VFDLFDEKKN GVIEFEEFIH ALSVFHPYAS IQEKTDFAFR LYDLRQTGFI EREEVQQMVS AILLESDMML SDELLTMIID KTFADADSDK DGKISKDEWN VYVHKHPSLL KNMTLPYLKD VTTAFPSFIF NTEVED
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Mediates salt tolerance, but only when phosphorylated. Competes with CIPK23 for a direct binding to AKT1, negatively regulating its activity via a protein kinase-independent mechanism.
Gene References into Functions
  1. CBL10 is critical for reproductive development in the presence of salt and that it functions in different pathways during vegetative and reproductive development. PMID: 26979332
  2. CBL10 negatively affects tolerance to drought and is not mediated by abscisic acid-induced signaling. Ca(2+) signaling through CBL10 differently affects the response to abiotic stresses, partly by regulating BR sensitivity of plant tissues. PMID: 26795150
  3. mutation of SCABP8 suppresses the ant-knockout salt-tolerant phenotype, implying that ANT functions as a negative transcriptional regulator of SCABP8 upon salt stress. PMID: 26054800
  4. The study revealed a CBL-interacting protein kinase-independent regulatory mechanism of calcineurin B-like proteins in which CBL10 directly regulates AKT1 activity and affects ion homeostasis in plant cells. PMID: 23331977
  5. SCABP8 and SOS3 are only partially redundant in their function, and each plays additional and unique roles in the plant salt stress response. PMID: 17449811
  6. findings suggest that CBL10 and CIPK24 (SOS2) constitute a novel salt-tolerance pathway that regulates the sequestration/compartmentalization of Na(+) in plant cells PMID: 17825054
  7. SCaBP8 phosphorylation is required for the activation of SOS1 and has a role in regulating salt tolerance in Arabidopsis. PMID: 19448033

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Subcellular Location
Vacuole membrane. Endosome. Cell membrane.
Protein Families
Calcineurin regulatory subunit family
Tissue Specificity
Expressed in shoots, leaves, stems, flowers and siliques. Barely detected in roots.
Database Links

KEGG: ath:AT4G33000

STRING: 3702.AT4G33000.1

UniGene: At.31618

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