CNGC18 Antibody

Code CSB-PA878476XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CNGC18 Polyclonal antibody
Uniprot No.
Target Names
CNGC18
Alternative Names
CNGC18; At5g14870; T9L3_170; Cyclic nucleotide-gated ion channel 18; Cyclic nucleotide- and calmodulin-regulated ion channel 18
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CNGC18 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cyclic nucleotide-gated ion channel required for directional pollen tube growth into the transmitting tract. Acts as a Ca(2+)-permeable divalent cation-selective channel inhibited by either lanthanum or gadolinium. Regulated by CPK32 to mediate Ca(2+) transport across the plasma membrane in response to Ca(2+) oscillation.
Gene References into Functions
  1. Data demonstrate that CNGC18 plays essential roles in pollen germination and tube growth as a Ca(2+) channel in Arabidopsis. PMID: 27322818
  2. Among 8 Ca(2+) channels, CNGC18 was the only one essential for pollen tube guidance. Its transmembrane domains are needed for this function. Point mutations R491Q or R578K resulted in abnormal Ca(2+) gradients and strong pollen tube guidance defects. PMID: 26929345
  3. Arabidopsis CNGC18 is a Ca(2)-permeable channel PMID: 24380879
  4. CNGCs have a widespread role in regulating cell-growth dynamics in both plant and animals PMID: 17726111
Subcellular Location
Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein.
Protein Families
Cyclic nucleotide-gated cation channel (TC 1.A.1.5) family
Tissue Specificity
Expressed in pollen grains. Not detected in leaves, roots or root hairs.
Database Links

KEGG: ath:AT5G14870

STRING: 3702.AT5G14870.1

UniGene: At.31874

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