Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Cp190 Polyclonal antibody

Uniprot No.

Q24478

Target Names

Cp190

Alternative Names

Cp190 antibody; E(mod)4-1 antibody; CG6384Centrosome-associated zinc finger protein CP190 antibody; Protein enhancer of mod(mdg4)4-1 antibody; dMAP190 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Cp190 protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may directly bind to insulator DNA at sites distinct from those recognized by su(Hw). Required during embryogenesis for axial expansion, an actin/myosin dependent process that distributes the dividing nuclei along the anterior-posterior axis of the syncytial embryo. Does not appear to play a crucial role in organizing centrosomal microtubules during mitosis.

Gene References into Functions

As binding of CP190 does not enhance luciferase activity in reporter assays. PMID: 24472778
we discuss the emerging role of CP190 in tethering genome, specifically in the perspective of insulator function in Drosophila. PMID: 23333867
The N-terminal of CP190 containing the BTB/POZ domain and the D-rich region is sufficient to mediate association with the insulator complexes. PMID: 21194420
CP190 is an essential protein, as flies homozygous for mutations in the Cp190 gene die at late pupal stages of development. PMID: 14996941
CP190 is a component of the gypsy chromatin insulator PMID: 15574329
CP190 has distinct nuclear and centrosomal functions, and it provides a crucial link between the centrosome/MT and actin/myosin cytoskeletal systems in early embryos. PMID: 16051175
Sumoylation does not affect the ability of CP190 and Mod(mdg4)2.2 to bind chromatin, but instead appears to regulate the nuclear organization of gypsy insulator complexes. PMID: 16628226
cis-regulatory elements, domain borders and promoters, the chromatin structure is dependent on CP190. PMID: 19229299

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Subcellular Location

Nucleus. Cytoplasm, cytoskeleton. Chromosome. Note=Nucleus in interphase. Colocalizes with other elements of the gypsy chromatin insulator complex at multiple sites on polytene chromosomes and at nuclear insulator bodies.

Tissue Specificity

Expressed in spermatids but not in mature spermatozoa. Localizes within the spermatids to a sheath of microtubules around the nucleus and to microtubules within the tail.

Database Links

KEGG: dme:Dmel_CG6384

STRING: 7227.FBpp0082580

UniGene: Dm.2423

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Cp190 Antibody

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