Product Details

Purity

>85% (SDS-PAGE)

Target Names

Cp190

Uniprot No.

Q24478

Alternative Names

Cp190; E(mod)4-1; CG6384Centrosome-associated zinc finger protein CP190; Protein enhancer of mod(mdg4)4-1; dMAP190

Species

Drosophila melanogaster (Fruit fly)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may directly bind to insulator DNA at sites distinct from those recognized by su(Hw). Required during embryogenesis for axial expansion, an actin/myosin dependent process that distributes the dividing nuclei along the anterior-posterior axis of the syncytial embryo. Does not appear to play a crucial role in organizing centrosomal microtubules during mitosis.

Gene References into Functions

As binding of CP190 does not enhance luciferase activity in reporter assays. PMID: 24472778
we discuss the emerging role of CP190 in tethering genome, specifically in the perspective of insulator function in Drosophila. PMID: 23333867
The N-terminal of CP190 containing the BTB/POZ domain and the D-rich region is sufficient to mediate association with the insulator complexes. PMID: 21194420
CP190 is an essential protein, as flies homozygous for mutations in the Cp190 gene die at late pupal stages of development. PMID: 14996941
CP190 is a component of the gypsy chromatin insulator PMID: 15574329
CP190 has distinct nuclear and centrosomal functions, and it provides a crucial link between the centrosome/MT and actin/myosin cytoskeletal systems in early embryos. PMID: 16051175
Sumoylation does not affect the ability of CP190 and Mod(mdg4)2.2 to bind chromatin, but instead appears to regulate the nuclear organization of gypsy insulator complexes. PMID: 16628226
cis-regulatory elements, domain borders and promoters, the chromatin structure is dependent on CP190. PMID: 19229299

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Subcellular Location

Nucleus. Cytoplasm, cytoskeleton. Chromosome. Note=Nucleus in interphase. Colocalizes with other elements of the gypsy chromatin insulator complex at multiple sites on polytene chromosomes and at nuclear insulator bodies.

Tissue Specificity

Expressed in spermatids but not in mature spermatozoa. Localizes within the spermatids to a sheath of microtubules around the nucleus and to microtubules within the tail.

Database Links

KEGG: dme:Dmel_CG6384

STRING: 7227.FBpp0082580

UniGene: Dm.2423

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Recombinant Drosophila melanogaster Centrosome-associated zinc finger protein CP190 (Cp190), partial

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