phm Antibody

Code CSB-PA894748XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) phm Polyclonal antibody
Uniprot No.
Target Names
phm
Alternative Names
phm antibody; Cyp306a1 antibody; CG6578Cytochrome P450 306a1 antibody; CYPCCCVIA1 antibody; EC 1.14.-.- antibody; Protein phantom antibody; Dmphm antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) phm protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the metabolism of insect hormones; responsible for ecdysteroid C25-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.
Gene References into Functions
  1. Phantom, a cytochrome P450 enzyme essential for ecdysone biosynthesis, plays a critical role in the control of border cell migration in Drosophila. PMID: 24373956
  2. CYP306A1 functions as a carbon 25 hydroxylase and has an essential role in ecdysteroid biosynthesis during insect development PMID: 15197185
  3. The Drosophila gene phantom (phm)encodes the microsomal 25-hydroxylase (Cyp306a1); strong expression of phm is restricted to the prothoracic gland cells of the Drosophila larval ring gland, and in the adult ovary, but also in the embryonic epidermis. PMID: 15350618
Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Protein Families
Cytochrome P450 family
Tissue Specificity
First seen at the early (syncytial) blastoderm stage 4. During cellularization of the blastoderm (stage 5), stripes of expression appear and remain through to stage 10. Expression becomes undetectable during germ band retraction (stages 11-14). By stage 1
Database Links

KEGG: dme:Dmel_CG6578

STRING: 7227.FBpp0074374

UniGene: Dm.5302

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