Recombinant Drosophila melanogaster Cytochrome P450 306a1 (phm), partial

Code CSB-YP894748DLU
MSDS
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Source Yeast
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Code CSB-EP894748DLU
MSDS
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Source E.coli
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Code CSB-EP894748DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP894748DLU
MSDS
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Source Baculovirus
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Code CSB-MP894748DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
phm
Uniprot No.
Alternative Names
phm; Cyp306a1; CG6578Cytochrome P450 306a1; CYPCCCVIA1; EC 1.14.-.-; Protein phantom; Dmphm
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the metabolism of insect hormones; responsible for ecdysteroid C25-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.
Gene References into Functions
  1. Phantom, a cytochrome P450 enzyme essential for ecdysone biosynthesis, plays a critical role in the control of border cell migration in Drosophila. PMID: 24373956
  2. CYP306A1 functions as a carbon 25 hydroxylase and has an essential role in ecdysteroid biosynthesis during insect development PMID: 15197185
  3. The Drosophila gene phantom (phm)encodes the microsomal 25-hydroxylase (Cyp306a1); strong expression of phm is restricted to the prothoracic gland cells of the Drosophila larval ring gland, and in the adult ovary, but also in the embryonic epidermis. PMID: 15350618
Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Protein Families
Cytochrome P450 family
Tissue Specificity
First seen at the early (syncytial) blastoderm stage 4. During cellularization of the blastoderm (stage 5), stripes of expression appear and remain through to stage 10. Expression becomes undetectable during germ band retraction (stages 11-14). By stage 1
Database Links

KEGG: dme:Dmel_CG6578

STRING: 7227.FBpp0074374

UniGene: Dm.5302

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