cpxR Antibody

Code CSB-PA360074XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) cpxR Polyclonal antibody
Uniprot No.
Target Names
cpxR
Alternative Names
cpxR antibody; yiiA antibody; b3912 antibody; JW3883Transcriptional regulatory protein CpxR antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) cpxR protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA. Required for efficient binding of stationary phase cells to hydrophobic surfaces, part of the process of biofilm formation. Induced upon cell surface binding, subsequently induces genes it controls (cpxP, dsbA and spy, degP is only partially induced). Binds and activates transcription from the degP promoter; binding is enhanced by phosphorylation. This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E, as well as that of CpxP protein. Other downstream effectors may include SrkA.
Gene References into Functions
  1. Activation of CpxR upregulates the expression of genes encoding protein folding and chaperone factors and downregulates the expression of a number of envelope proteins/ PMID: 29311237
  2. X-ray structures of the CpxR reveals allosteric switching mechanism, according to which CpxR binding to one CpxA protomer triggers autophosphorylation in the second protomer. PMID: 28552574
  3. findings show that fine-tuning the Cpx stress response is critical to maintain cell wall homeostasis, affecting cell growth, morphology, division, and antibiotic resistance PMID: 26908573
  4. The data lend strong support to the hypothesis that acetyl phosphate has a direct effect on transcription regulation in E. coli via phosphorylation of CpxR, and that RNA polymerase acetylation can modulate this response. PMID: 26790713
  5. Data suggest that the cpxA/cpxR signaling system clearly depends on lipid bilayer properties; increase in anionic surface charge of lipid vesicles results in increased phosphorylation of cpxR. PMID: 25993101
  6. Data indicate that activation of the CpxR/CpxA system stimulates multiple antibiotic resistance (mar) transcription, suggesting a new regulatory circuit that enhances the multidrug resistance cascade. PMID: 25294881
  7. A cpxA mutant lacking its phosphatase activity results in constitutive activation of its cognate response regulator, CpxR, and fosfomycin resistance. PMID: 24163343
  8. overexpression represses transcription of the ung gene (uracil-DNA glycosylase) and ultimately leads to the induction of mutation. PMID: 15576781
  9. findings demonstrate that the Cpx (sensor kinase CpxA and the response regulator CpxR ) response is also involved in the expression of the type IV bundle-forming pili of enteropathogenic E. coli (EPEC). PMID: 15629938
  10. biochemical analysis of the CpxRAP envelope stress system of Escherichia coli PMID: 17259177
  11. This study investigated the role of the Cpx response in enteropathogenic Escherichia coli type III secretion through the locus of enterocyte effacement pathogenicity island expression. PMID: 18227171
  12. The CpxA and CpxR regulated genes that played role in envelope protein misfolding and biofilm formation. PMID: 19103922
  13. MalE mutant causes elevated levels of phosphorylated CpxR and it does not affect the phosphorylation level of CpxA. PMID: 19596441

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Subcellular Location
Cytoplasm.
Database Links
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