Recombinant Escherichia coli Transcriptional regulatory protein CpxR (cpxR)

Code CSB-YP360074ENV
MSDS
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Source Yeast
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Code CSB-EP360074ENV
MSDS
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Source E.coli
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Code CSB-EP360074ENV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP360074ENV
MSDS
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Source Baculovirus
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Code CSB-MP360074ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cpxR
Uniprot No.
Alternative Names
cpxR; yiiA; b3912; JW3883Transcriptional regulatory protein CpxR
Species
Escherichia coli (strain K12)
Expression Region
1-232
Target Protein Sequence
MNKILLVDDD RELTSLLKEL LEMEGFNVIV AHDGEQALDL LDDSIDLLLL DVMMPKKNGI DTLKALRQTH QTPVIMLTAR GSELDRVLGL ELGADDYLPK PFNDRELVAR IRAILRRSHW SEQQQNNDNG SPTLEVDALV LNPGRQEASF DGQTLELTGT EFTLLYLLAQ HLGQVVSREH LSQEVLGKRL TPFDRAIDMH ISNLRRKLPD RKDGHPWFKT LRGRGYLMVS AS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA. Required for efficient binding of stationary phase cells to hydrophobic surfaces, part of the process of biofilm formation. Induced upon cell surface binding, subsequently induces genes it controls (cpxP, dsbA and spy, degP is only partially induced). Binds and activates transcription from the degP promoter; binding is enhanced by phosphorylation. This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E, as well as that of CpxP protein. Other downstream effectors may include SrkA.
Gene References into Functions
  1. Activation of CpxR upregulates the expression of genes encoding protein folding and chaperone factors and downregulates the expression of a number of envelope proteins/ PMID: 29311237
  2. X-ray structures of the CpxR reveals allosteric switching mechanism, according to which CpxR binding to one CpxA protomer triggers autophosphorylation in the second protomer. PMID: 28552574
  3. findings show that fine-tuning the Cpx stress response is critical to maintain cell wall homeostasis, affecting cell growth, morphology, division, and antibiotic resistance PMID: 26908573
  4. The data lend strong support to the hypothesis that acetyl phosphate has a direct effect on transcription regulation in E. coli via phosphorylation of CpxR, and that RNA polymerase acetylation can modulate this response. PMID: 26790713
  5. Data suggest that the cpxA/cpxR signaling system clearly depends on lipid bilayer properties; increase in anionic surface charge of lipid vesicles results in increased phosphorylation of cpxR. PMID: 25993101
  6. Data indicate that activation of the CpxR/CpxA system stimulates multiple antibiotic resistance (mar) transcription, suggesting a new regulatory circuit that enhances the multidrug resistance cascade. PMID: 25294881
  7. A cpxA mutant lacking its phosphatase activity results in constitutive activation of its cognate response regulator, CpxR, and fosfomycin resistance. PMID: 24163343
  8. overexpression represses transcription of the ung gene (uracil-DNA glycosylase) and ultimately leads to the induction of mutation. PMID: 15576781
  9. findings demonstrate that the Cpx (sensor kinase CpxA and the response regulator CpxR ) response is also involved in the expression of the type IV bundle-forming pili of enteropathogenic E. coli (EPEC). PMID: 15629938
  10. biochemical analysis of the CpxRAP envelope stress system of Escherichia coli PMID: 17259177
  11. This study investigated the role of the Cpx response in enteropathogenic Escherichia coli type III secretion through the locus of enterocyte effacement pathogenicity island expression. PMID: 18227171
  12. The CpxA and CpxR regulated genes that played role in envelope protein misfolding and biofilm formation. PMID: 19103922
  13. MalE mutant causes elevated levels of phosphorylated CpxR and it does not affect the phosphorylation level of CpxA. PMID: 19596441

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Subcellular Location
Cytoplasm.
Database Links
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